The Proliferation And Mechanism Of Extract From Sculellaria Barbata And Oldenlandia Diffusa Resisting Lewis Lung Carcinoma Cell

Lung cancer is the most common cancer in our country,its morbidity and mortality are in the forefront of all cancers.Nowadays most of chemotherapy drugs for the treatment cancer are western medicine composition,and its serious side effects restricts the use of western medicine,so now more and more research shifted to Chinese medicine,flavonoids is the nature of bioactive substances that widely exist in the world,the present study showed that flavonoids can antioxidant,prevent blood vessel growth,anti-inflammatory,antiviral,fall blood sugar and blood fat,resist osteoporosis and other pharmacological effects.Barbed skullcap herb,Oldenlandia diffusa as traditional detoxification medicine,its main active ingredients such as flavonoids and sterols.Barbed skullcap herb,Oldenlandia diffusa in LLC in mouse models of lung cancer antitumor situation was not reported.First by orthogonal design method of optimizing Barbed skullcap herb,Oldenlandia diffusa extraction technology of flavonoids.Flavonoids content as the index,by single factor and orthogonal experiment,the method of combining with extraction temperature,extraction time and solid-liquid ratio and extraction times,ethanol volume fraction to examine factors,selected the best extraction technology of spreading hedyotis herb:extraction temperature 80?,extraction 120min each time,2 times,and liquid ratio of 1:20,ethanol volume fraction is 70%,under the condition of the extraction of extraction rate can reach 16.61%.And then to extract total flavonoids ethyl acetate extraction,flavonoids content is 20.04%.Second,LLC cells according to 0.2 mL(cell content of 2×106/mL)in C57BL/6 mice right axillary subcutaneous,lung cancer in mice model was established,according to body weight in mice were randomly divided into model group,drug delivery with Barbed skullcap herb,Oldenlandia diffusa extracts from medium,low,medium and high dose lavage daily dosage,measuring mice tumor length,width,calculation of tumor volume.Results showed that in these fields,spreading extractive can effectively inhibit the growth of tumor in mice,the inhibition rate is respectively:low doses 31.64%,medium dose 32.76%,high dose 68.36%.Barbed skullcap herb,Oldenlandia diffusa ethyl acetate layer can improve the spleen index and thymus index,the effect of extracttive on antitumor effect may be related to improve the body's immune.The result of HE staining show there is destructive of extractive on tumor tissue,compared with model group,medication group a large area of tumor cells in tumor tissue necrosis area,tumor cell morphology significantly damaged.Secondly by UPLC identify scutelarin,luteolin,apigenin.By MTT experiment to investigate scutelarin,luteolin and apigenin's effect on LLC cell.Results showed that scutelarin 20 uM-200 uM has the inhibition on the LLC cells and the concentration dependence,and range in 80-200uM have significant differences,IC50 alue concentration of 184.5 vM,luteolin 10-200 uM has the inhibition on the LLC cells,and the concentration dependence of 20-200uM has an inhibitory effect and its have concentration dependence and IC50 is 43.69 uM.Apigenin at 10-200 uM has the inhibition on the LLC cells,and the concentration dependence of 50-200 uM has an inhibitory effect and its IC50 is 76.95 uM.To detect the effect of scutelarin,luteolin and apigenin on LLC cells,Western blot is used for the mechanism of three compound on LLC cells.The rult shows that MAP4K3 protein expression has been reduced,expression of LC-3 protein is higher,and expression of P62 protein is reduced,and then detected using creep plates chek autophagy protein fluorescence aggregation,and result shows that it works on LLC cells may be autophagy.Lung cancer in mice model is established,according to body weight in mice were randomly divided into model group,drug delivery,with scutellarin low,medium and high dose lavage daily dosage,measuring mice tumor length,width,calculation of tumor volume.Results showed that in these fields,spreading scutellarin can effectively inhibit the growth of tumor in mice,the inhibition rate is respectively:low doses of 29.01%,dose of 50.62%,high dose of 80.34%.And the result show scutellarin can improve the spleen index and thymus index,indicate the effect of scutellarin on tumor-bearing mice may be related to improve the body's immune.HE staining shows the tumor tissue is destroyed that compared with model group,threre is a large area of tumor cells destruction in tumor tissue,tumor cell morphology are also significantly damaged.