Study On The Effect Of Hedyotis Diffusa And Scutellaria Barbata Extracts On Apoptosis Of Tongue Squamous Cell Carcinoma Cal-27 Cell

Objective:This study was conducted to investigate the effects of Hedyotis diffusa extract and Sculellaria barbata extract on the proliferation and apoptosis of human tongue squamous cell carcinoma Cal-27 cells.Methods: The effects of the extracts of Hedyotis diffusa and Sculellaria barbata on the proliferation of Cal-27 cells were determined by CCK-8 method.Annxin V-FITC/PI double staining flow cytometry was used to detect the effects of Hedyotis diffusa and Sculellaria barbata extracts on the apoptosis of Cal-27 cells.The protein expression levels of Caspase-3,Caspase-8,Caspase-9 and Caspase-12 in Cal-27 cells treated with the extracts of Hedyotis diffusa and Sculellaria barbata were determined by Western Blot.Results: CCK8 experiment showed that,compared with Control group,0.1mg/m L cell proliferation inhibition rate was 2.44±0.36% after 24 h treatment,and the difference was not statistically significant(P > 0.05).The inhibition rates of cell proliferation of 0.25mg/m L,0.5mg/m L,1mg/m L,2.5mg/m L and5mg/m L were 8.63±1.1%,21.62±2.01%,41.91±2.27%,62.66±1.48% and72.33±1.61%,respectively.The difference was statistically significant(P <0.05).The results indicated that the proliferation inhibition rate of Cal-27 cells treated with HD and SB aqueous extracts increased with time.Annexin V-FITC/PI double staining flow cytometry showed that the apoptosis rates of0.25mg/m L,0.5mg/m L and 1mg/m L were 9.84±0.19%,16±1.65% and21.64±0.75% after 24 h treatment compared with the Control group.The difference was statistically significant(P < 0.05).The results indicated that the apoptosis rate of Cal-27 cells treated with HD and SB water extract increased with time,and the difference was statistically significant(P < 0.05).Compared with Control group,the expression levels of Caspase-3,Caspase-8,Caspase-9,Caspase-12 after HD and SB water extract treatment were significantly increased,and the differences were statistically significant(P < 0.05).Conclusion:The aqueous extracts of Hedyotis diffusa and Sculellaria barbata inhibited the proliferation of Cal-27 cells and promoted the apoptosis of Cal-27 cells.The inhibition rate of proliferation and apoptosis rate were proportional to the drug concentration.It also promoted the apoptosis of Cal-27 cells by increasing the expression of Caspase-3,Caspase-8,Caspase-9 and Caspase-12 proteins.