Analysis Of Nasal Flora Distribution Of Chronic Sinusitis By 16S RDNA High-throughput Sequencing

Objective: The aim of this study was to analyze the characteristics of nasal flora of CRSs NP(chronic rhinosinusitis without nasal polyposis)and control groups by using 16 S r DNA high-throughput sequencing.We want to explore new methods for the diagnosis of chronic sinusitis,and to provide new ideas for the prevention and treatment of chronic sinusitisMethods: 13 Patients with chronic sinusitis without nasal polyps(CRSs NP)meeting the diagnostic criteria and 5 normal patients were selected and labeled as patient group and control group.Nasal Swab was used to collected in secretions of middle meatus in both groups as specimens,from which sample DNA was extracted,and amplified 16 S r DNA V4-V5 by PCR and sequenced by Mi Seq Illumina platform.The nasal microbial diversity and structure analysis were analyzed by mother and other software.Results: A total of 597 OUTs were detected from 18 samples,belonging to 21 phylum(control group : 16,patient group : 20)and 233 genus(control group : 149,patient group:212),some of them,which the prevalence is more than 1%,were called dominant phylum(control group:4,patient group:6)and dominant genus(control group:15,patient group:20)。The sequencing showed no significant difference in bacterial abundance and diversity among patients in patient and control groups.Among the dominant phylum,Proteobacteria,Firmicutes,Antinobacteria and Bacteroidetes have a common existence in each group,while among the dominant genus,Staphylococcus,Sphingomonas,Propionibacterium,Sphingomonas and Dolosigranulum have a common existence in each group.The differences between the dominant bacteria phylum: Firmicutes and Fusobacteria had higher abundance in patient group(P<0.05),and Proteobacteria was higher in control group(P<0.05).Compared with control group,the abundance of Staphylococcus,Streptococcus,Fusobacterium,Corynebacterium,Anaerococcus and Actinomyce was higher in patient group in the dominant genara(P<0.05),while that of the Stenotrophomonas,Bacillus,Pandorea was lower at phylum level(P <0.05).Differences among dominant species: Prevotella_melaninogenica was significantly increased in patient group.(P <0.05)Conclusion: The etiology of CRS is complicated,and the pathogenic bacteria are complex and diverse.By using 16 S r DNA high-throughput sequencing,various microbial populations can be accurately detected,and the distribution of nasal microflora in patient and control populations is analyzed.There was a certain correlation between the increase of Firmicutes,Fusobacteria,the decrease of Proteobacteria and the incidence of CRS.We conclude that CRS changed the nasal microflora and bacterial diversity,and how to maintain the balance of nasal microecology will provide new insights into the treatment of CRS.