Study On The Toxicity And Mechanism Of The Main Toxic Components In Jatropha Curcas Kernel Cake

Jatropha curcas L.is used as the main tree for producing biodiesel and is widely planted because of its high yield and rich seed oil,it,.It produces large number of Jatropha curcas cake which is the by-product after refining biodiesel,Jatropha curcas cake contains a variety of nutrients,which is a high quality potential protein feed,but on account of its has strong toxicity,its development and utilization is restricted.In addition,due to the lack of understanding its toxic mechanism,there is no good strategy to cope with human and animal consumption.The purpose of this study is to study the toxicity and toxicity mechanism of the toxic substances in the early separation and purification,and to provide theoretical basis for the development of detoxification technology.At the same time,it also provides a reference for the development and utilization of the toxic components.Through the early stage research in our laboratory,the toxic substances chem2 in the Jatropha curcas cake were purified.The chemical nature of chem2 was12-hydroxy-(10E)-octadecenoic acid and 9-hydroxy-(10E)-octadecenoic acid.In this study,the physiological and pathological toxicity and molecular mechanism of chem2 were observed at molecular level,cell level and animal tissue level.At the animal level,the following experiment were applied,skin toxicity test,tail vein injection experiment,tail subcutaneous injection experiment,smooth muscle tension experiment,nd gastric lavage experiment.at the cell level,the following experiments were performed as coculturing chem2 with human breast cancer cell lines(MCF-7),rat myoblasts(L6)and HepG2,Coagulation experiment,LAD2 Degranulation experiment.At the molecular level,the mRNA expression level was used to understand the conjugated protein-3(UCP-3).Detection of protein kinase C(PKC)expression level by western-blot and the active oxygen levels of MCF-7 cell lines were detected.Results showed that Chem2 had strong skin toxicity,which could lead to inflammatory reaction and injury in the skin.After injection of chem2 in the caudal vein,liver,lungs and other organs,it would be filled in blood and resulted bleedingand the death of mice.The subcutaneous injection of chem2 in the tail could lead to subcutaneous tissue necrosis,which resulting in a broken tail.Chem2 increased the contraction of the smooth muscle and the tension of the smooth muscle.Gastric infusion with chem2 could cause diarrhea in mice.Chem2 had a strong catalytic effect on the proliferation of MCF-7.The proliferation of L6 was promoting too,but not as strong as MCF-7.Chem2 could promote the perforation of HepG2.Adding chem2 in blood would significantly boost blood clotting.Chem2 significantly increased the degree of degranulation after the stimulation of LAD2.The expression of UCP-3 and PKC enzyme was significantly reduced after cultured with the complete culture of chem2.Chem2 increased the reactive oxygen level of McF-7 that had a concentration dependence.Conclusion: Chem2 has a strong toxic effect.Chem2 promotes ROS generation,reduces UCP-3 mRNA expression levels and PKC expression levels,which are important components of its toxic mechanism.