Research The Association Between Hepatocyte Structure And Cell Growth In Three-dimensional Cell Printing Technique

Liver tissue has complex choroid structure.Studying the correlation between the structure of artificial liver tissue and cell growth is helpful to optimize the design of liver tissue engineering scaffold,improve the success rate of artificial liver tissue,and promote the development of liver tissue engineering technology.The results of the traditional tissue engineering scaffold preparation method and preparation process are closely linked,it is difficult to control the scaffold structure characteristics,biological 3D printing through computer aided design and manufacturing precise preparation of complex structure organization,implementation provides a solution for the design of related tissue engineering,the association of support structure with cell growth,functional reconstruction provides precise control of possible structure.In this paper,combined with the anatomical features of bionic liver unit design 16 liver unit model structure,analyzes the quantitative association of different design structure and cell growth in a fluid environment using CFD simulation to determine the effect of key structural features of cell growth;then cell 3D printing technology in vitro constructed 6 kinds of artificial liver tissue based on using optical coherence tomography(OCT)to complete the quantitative structure artificial liver tissue characterization by fluorescence microscopy and cell proliferation assay to quantify cell growth state,so as to establish a quantitative correlation model between 3D liver tissue structure and cell growth in print from two aspects of simulation and experiment.The following is an introduction to the contents of each chapter:(1)in the first chapter,we summarized the liver tissue engineering and cell 3D printing technology,summarized the three elements of liver tissue engineering and the advantages and disadvantages of different cell 3D printing technology.The research on the relationship between scaffold structure and cell growth at home and abroad was investigated to provide reference for the construction of liver cell in this article.(2)the related contents of human liver were introduced,and the basic unit of liver tissue,the structure and function of hepatic lobule were introduced.The study on the liver unit modeling at home and abroad in the field of liver tissue engineering,comparing different modeling methods the advantages and disadvantages of the proposed model for the preparation of the liver cell,liver cell modeling including structure design,manufacturing process,seed cells,matrix material selection.At last,the CFD simulation technology and its application in the field of organization engineering are introduced.(3)based on the bionic principle,the liver cell model of sixteen structures was designed andthe CFD simulation was carried out.The numerical simulation results show that the flow velocity in the liver cell is obviously influenced by the pore size,and the larger the pore flow is,the larger the pore flow is.Compared with the square and hexagon,the permeability and flow rate of the triangular pore liver unit with the same pore size is the smallest,and the ability to allow the fluid to pass is the weakest.In addition,it is found that the design of the lateral channel for the liver cell model can increase the flow velocity of more than 25% in the micropipeline,and more than 35% of the total fluid flow.(4)based on cell 3D printing technology to complete the preparation of liver cells,followed by OCT technology of 3D printing cells loaded hydrogel liver unit model structure quantification,the experimental results show that the better stability of square pore structure and large pore size liver unit printing,printing results closer than the preset value;triangle pore structure square,the pore structure can improve the liver unit porosity of 9%.Besides,the mechanical design of the support with side channel is poor,so it can't print samples exactly according to the preset structure,but the structure can increase the porosity of the liver unit 10% and the specific surface area of 9%.Combined with biological experiments,it is found thatIn conclusion,it is feasible to use cell 3D printing technology to prepare cell loaded hydrogel liver cell model.Combined with CFD simulation and OCT technology,we can perform good performance characterization for 3D model design and printing.In this paper,we studied the fluid characteristics and parameters of different structural liver units,summarized the three-dimensional model structure suitable for hepatocyte growth,and provided a theoretical basis for building better liver cell models in the future.