Experimental Research Of The Olfactory Receptor Neurons Of Olfactory Dysfunction By Allergic Rhinitis

Objective:To set up an intranasal ovalbumin-induced animal model of allergic rhinitis(AR)accompanied with olfactory dysfunction in mice.By observing the olfactory pathway in mice by manganese-enhanced magnetic resonance imaging(MEMRI)and the relatively morphologic,structural and immunological changes in olfactory epithelium,to discuss the influence of AR over olfactory receptor neurons(ORNs).Methods:Forty SD mice were randomly divided into two groups,the research group(n=30)and the control group(n=10).The research group was intraperitoneally injected and intranasal application of ovalubumin to establish an allergic rhinitis mice model.The olfactory function of the mice was evaluated by buried food test(BFT).ELISA was performed to measure the level of IgE in serum.MEMRI images were acquired with a 7.0 T micro-MR scanner.HE dyeing and immunohistochemistry were used to observe the tissues morphology change of olfactory mucosa and OMP expression.Results:The olfactory function evaluation of AR mice model indicated that the incidence rate of olfactory dysfunction in allergic rhinitis mice was 40.0%.The olfactory epithelium became thinner,layer numbers of ORNs were decreased with disorder arrangement and the OMP expression was decreased much more in AR with olfactory dysfunction mice than those in AR without olfactory dysfunction(p=0.018)and the control group(p=0.0141).Conclusion:An animal model of allergic rhinitis accompanied with olfactory dysfunction in mice was successfully established.The influence of AR over ORNs and thus cause the change of the olfactory pathway is one of the major pathogenesis of olfactory dysfunction in allergic rhinitis.