| 1.Objective:This study examined the levels of circulating smooth muscle progenitor cells(SPCs),transforming growth factor beta 1(TGF-betal),high density lipoprotein(HDL)and low density lipoprotein(LDL)subtype in coronary heart disease(CHD)patients with blood stasis syndrome(BSS).To provide reference and basis for the clinical diagnosis and pathological mechanism of CHD with BSS.2.method:91 CHD patients who were hospitalized in Department of Cardiology of Guang' anmen Hospital,China Academy of Chinese medicine sciences from September 2017 to February 2018 were selected.They were divided into BSS group and non-BSSgroupaccording to TCM syndrome differentiation.30 healthy volunteers from Guanganmen hospital and south district were used as control group at the same time.The level of SPCs in human peripheral blood was detected by flow cytometry.The concentration of TGF-? 1 in serum was detected by ELISA.The distribution and level of HDL subtypes and LDL subtypes in plasma were detected by lipoprotein classification detection system(lipoprint system).The above indexes were compared between CHD group and healthy group,and between BSS group and non-BSS group.Statistical analysis:using the Excel table to collect data,SPSS23.0 statistics software for statistical analysis.The measurement data were described with mean standard deviation(x±s).The test is used for normal distribution,and the variance test is used when the normal distribution is conformed to the homogeneity of variance,and the nonparametric test is used in the nonnormal distribution.Counting data are described with frequency and composition ratio,and chi square test is used for comparison between groups.When P<0.05 was set,the difference was statistically significant.3.Results:3.1 The levels of SPCs and TGF-betal in peripheral blood in CHD patients.Compared with healthy group,the level of SPCs in CHD group was increased,the difference was statistically significant(p<0.01),and the level of TGF-beta 1 was increased,the difference was statistically significant(p<0.05).3.2 The distributions and levels of HDL subtypes and LDL subtypes in peripheral blood in CHD patients.3.2.1 The levels of lipid in CHD patientsCompared with healthy group,the levels of CHO,LDL,APoAl and APoB were increased in CHD group,the difference was statistically significant(p<0.01).3.2.2 The distribution of HDL subtypes of peripheral blood in patients with CHD.Compared with healthy group,HDL3%in CHD group was reduced,the difference was statistically significant(p<0.05);HDL7%,HDL8%,HDL9%,Small(8-10)%proportion increased,the differences were statistically significant(p<0.01)3.2.3 The level of HDL subtypes of peripheral blood in patients with CHD.Compared with healthy group,HDL6,HDL7,HDL8,HDL9,Intermediate(4-7)and Small(8-10)were increased in CHD group,and the differences were statistically significant(p<0.01 or p<0.05).3.2.4 The distribution of LDL subtypes of peripheral blood in patients with CHD.Compared with healthy group,LDL1%in the CHD group was reduced,the difference was significant(p<0.01);LDL3%was increased,the difference was statistically significant(p<0.01).3.2.5 The level of LDL subtypes of peripheral blood in patients with CHD.Compared with healthy group,LDL1 and MeanLDLsize(A)were reduced in CHD group,the differences were statistically significant(P<0.01),LDL3 and LDL4 were increased,the differences were statistically significant(p<0.01).3.3 The levels of SPCs and TGF-beta 1 in peripheral blood in BSS patients with CHD.Compared with non-BSS group,the levels of SPCs and TGF-beta 1 in BSS group were significantly higher,and the differences were statistically significant(p<0.01).3.4 The distributions and levels of HDL subtypes and LDL subtypes in peripheral blood in CHD patients with BSS.3.4.1 The levels of lipid in CHD patients with BSS.Compared with CHD patients with non-BSS group,CHO,LDL-C and ApoB were increased in CHD patients with BSS,the differences were statistically significant(p<0.01).3.4.2 The distribution of HDL subtypes of peripheral blood in CHD patients with BSS.Compared with CHD patients with non-BSS group,HDL2%,Large(1-3)%were reduced in CHD patients with BSS,the differences were statistically significant(P<0.05),HDL7%,HDL8%,HDL10%,Small(8-10)%were increased,the differences were statistically significant(P<0.05),HDL6%.was increased,the difference was statistically significant(P<0.01).3.4.3 The level of HDL subtypes of peripheral blood in CHD patients with BSS.Compared with CHD patients with non-BSS group,HDL2 was decreased in CHD patients with BSS,the difference was statistically significant(p<0.05),HDL8,Small(8-10)were increased,the differences were statistically significant(p<0.05).3.4.4 The distribution of LDL subtypes of peripheral blood in CHD patients with BSS.Compared with CHD patients with non-BSS group,LDL1%was reduced,the difference was statistically significant(p<0.01),LDL3%was increased,the difference was statistically significant(p<0.01).3.4.5 The level of LDL subtypes of peripheral blood in CHD patients with BSS.Compared with CHD patients with non-BSS group,LDL1 MeanLDLsize(A)was reduced,the difference was statistically significant(p<0.01),LDL3 and LDL4 were increased,the differences were statistically significant(p<0.05).4.Conclusion:4.1 The levels of SPCs and TGF-beta 1 increased in peripheral blood in CHD patients.HDL3%decreased,HDL7%-HDL10%?HDL4-HDL10 increased.The average LDL particles and LDL1%decreased.LDL level increased,mainly in LDL3%?LDL3-LDL6.4.2 The levels of SPCs and TGF-beta 1 increased in peripheral blood in CHD patients with BSS.HDL1%-HDL3%?HDL2 decreased,HDL6%-HDL10%?HDL8-HDL10 increased.The average LDL particles and LDL1%?HDL1 decreased.LDL level increased,mainly in LDL3%?LDL3-LDL6. |
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