Functional And Biocompatibility Evaluation Of Absorbable Macroporous Polysaccharide Composite Based Biological Material

Carboxymethyl chitosan and gelatin have been widely used in food,cosmetics and pharmaceuticals industry.In this research,absorbable macroporous polysaccharide composite based biological material was comprised of carboxymethyl chitosan and gelatin.They were made by irradiation crosslinking by 60Co and then lyophilized.Carboxymethyl chitosan and gelatin show excellent biocompatibility,degradation and hemostasis ability.Biological hemostatic materials are one of indispensable biomedical materials in clinical application,while there are hardly any alternative species of versatile hemostatic materials currently.The hemostatic materials nowadays are more or less difficult to be degraded in body with poor hemostatic effect and inferior biocompatibility.This research aims to verify the prospect of absorbable macroporous polysaccharide composite based biological material for its clinical performance.We studied ts degradation,functional performances and biocompatibility both in vitro and in vivo.(1)To preliminaryly determine its functional ability,basic physical and chemical properties were examined.It was found absorbable macroporous polysaccharide composite based biological material has good physicochemical properties,the degree of deacetylation and carboxylation are 37.53%,36.50%respectively.Namely,carboxyl groups and amino groups contents in absorbable macroporous polysaccharide composite based biological material are high which benefit to their interaction with blood coagulation system to clot.It has excellent water absorption ability with the absorption amount about 71 times higher than its weight.The coagulation index and hemolysis ratio show it is of great biocompatibility.(2)Through the degradation experiments in vivo and vitro,the results show the absorbable macroporous polysaccharide composite based biological material has excellent degradation performance both in vivo and in vitro with the degradation rate about 19 mg·d-1 and 20 mg·d-1,respectively.(3)To verify its biocompatibility,cytotoxicity test,immunogenicity and subchronic toxicity experiments were developed.It demonstrates great biocompatibility.(4)To verify its functional ability in vivo,we developed new liver injury and thyroid resection model.Absorbable macroporous polysaccharide composite based biological material functions effectively on hemostasis in liver injury.Its hemostatic time was about 26 times lower while the amount of bleeding decreased by 18 times compared with control groups.Additionally,it can effectively reduce postoperative suction drainage in the thyroid resection model with a total reduction of 30.37%.In this research,it demonstrates absorbable macroporous polysaccharide composite based biological material under irradiation crosslinking has excellent functional ability,great biocompatibility and biodegradability;Not only can it be used in viscera bleeding,but also it can reduce postoperation suction drainage after resection of thyroid.It successively overcomes the shortcomings of the current biological hemostatic materials which can only be used for body surface and can't be degraded in vivo with poor biocompatibility.Furthermore,we widened the application range of absorbable macroporous polysaccharide composite based biological material in phamarceutical industry.This research can not only provide reference for researching the clinical use of other hemostasis materials but also has great significance for resolving the scarcity problem of hemostasis materials.