Study On The Changes Of Telomere Length From Peripheral Blood Cell In Patients With Acute Coronay Syndrome

[Background] In 2009,three American scientists,Elizabeth H.Blackburn,Carol W.Greider and Jack W.Szostak,Won the Nobel Prize for medicine with their study of ―how the telomere and the telomerase protect the chromosome‖.Telomere was considered as―the clock of molecular‖.In the early 1973,Olovnikov recognized the correlation between the loss of telomere ang the aging of the body,he considered that the loss of telomere caused by the problem terminal replication was likely to regulate the cell life span.According to the study of telomerase activity,Bodnar et al found that the reverse transcription function of telomerase can delay the lose of telomere length,then prolonged the life cycle of the human normal cells,these can strongly confirm the relationship between telomere and aging.Age is an independent risk factor for coronay heart disease.The pathogenesis of coronary heart disease is the atherosclerosis caused by intimal lipid precipitation.When the development of the aging,endothelial cell would senesce and injury,leading to lipid precipitation gradually and the atheromatous plaque formation.Furthermore,oxidative stress of endometrium and chronic inflammation caused by chronic diseases such as hypertension and diabetes,jointly promoted the progress of coronary heart disease.As the highest rate of CAD mortality,acute coronay syndrome age distribution become younger.Some studies found that except for the affection of diet and living habit,genetic predisposition was more important to cause the ACS.Therefore,using the ACS patients as the research object can more conducive to elucidate its genetic characteristics,and it is expected to be a new target or early detection,early diagnosis and early treatment of coronary heart disease.This study mainly measured the telomere length of peripheral blood of ACS patients,and analyzed the correlation between its length and the occurrence of disease.Pearson correlation was used to explore the relationship between the severity of coronary artery disease and telomere length.[Object] 1.Discussing the change rules of peripheral blood telomere length in ACS and compared with those of normal control groups.2.To explore whether the related risk factors of ACS are related to telomere length.[Method] According to the inclusion and exclusion criteria of this study,the peripheral blood samples of coronary angiography in the second affiliated hospital of guangzhou medical university were collected from 2017.8-2018.3.It contain cases of ACS,cases of normal people as negative control group.After the peripheral blood DNA was extracted from the kit,then performing real-time fluorescence quantitative PCR.Lastly,calculating its relative telomere length.All data of this study were analyzed by SPSS v16.0.The statistical methods is Independent Sample T test,Chi-squared Test,Mann-Whitney Test and Pearson regression analysis.[Results] In all the 74 patients,the average length T/S ratio in the control group was 1.36 + 0.63,and the average telomere length T/S ratio in the ACS group was 0.87 + 0.47,we can found that the telomere length in the control group was higher than that in the ACS group(P<0.05),which have have statistical significance.Two groups of risk factors,including hypertension history,history of diabetes,smoking,uric acid,cholesterol,triglyceride,total bilirubin,creatinine were not significantly different.Two groups of patients were divided into three age groups,and the telomere length of ACS was significantly shorter than that in the control group between 61-75 and 46-60 years,both of which were statistically significant.In the group with no more than 45 years of age,although the mean of telomere length in the ACS group was lower than that of the control group,there was no statistical significance.Pearsoncorrelation was used to investigate the severity of coronary artery disease with no significant correlation with telomere length.[Conclusion] 1.The relative telomere length of peripheral blood leukocytes in patients with ACS group was significantly shorter than that in the control group.