Effects Of T3 On Secretion Of Vascular Growth Factors By Maternal-Fetal Interface In Early Pregnancy

ObjectiveThyroid hormones(TH)are tyrosine iodides that are synthesized and secreted by the thyroid gland and mainly include tetraiodothyronine(T4)and triiodothyronine(T3).The physiological role of thyroid hormones is very wide,is to maintain the body's normal growth and development of essential hormones.Thyroid disease is a common endocrine disease,and women of childbearing age are the high incidence of thyroid diseases.The physiological changes of the pregnancy increase the burden of thyroid function and the corresponding physiological changes of the thyroid function.The changes in hormone levels caused by abnormal thyroid function further affect the process of pregnancy and the development of the fetus,and cause the bad pregnancy outcome.Abortion is a common obstetrics and gynecological disease,most of the abortion occurred in the first three months of pregnancy.About 50% of abortions are associated with chromosomal abnormalities(aneuploidy).However,the causes and mechanisms of the remaining 50% of early abortions are poorly understood.It has been reported in the literature that vascular dysfunction may play an important role in the pathological changes of abortion,of which about 70% of early abortion is related to the reduction of blood vessel formation and vascularization at the maternal-fetal interface.The association between thyroid dysfunction during pregnancy,especially hypothyroidism,decreased thyroxine secretion and pregnancy complications has been widely concerned.Abortion is a common complication in early pregnancy.Abnormal secretion of vascular growth factor is known to cause abortion,but the mechanism of abortion caused by abnormal thyroid function is unclear.The effect of thyroxine on the angiogenesis and development of placenta and placental endocrine activity is poorly understood.Therefore,it is hypothesized that abnormal thyroid function may change the expression of vascular growth factor at the maternal-fetal interface to influence the pregnancy process and outcome.Methods(1)Decidual and villi tissues were obtained in 12 cases of decidual and villi tissues from the Department of Obstetrics and Gynecology in Xijing Hospital,and the chorionic stromal cells And cultured with rabbit anti-prolactin antibody,mouse anti-vimentin antibody and rabbit anti-horny protein 7 antibody.The cells were identified by immunohistochemistry and cultured for primary cell culture.(2)Activated carbon was used as the control group and 0 nMol / L of T3-free activated carbon was used as the control group.The culture medium of activated carbon was used to add 1 nMol / L,10 nMol / L,100 nMol / L three concentrations of T3 experimental group.The detection of VEGF-A,Ang-1 and Ang-2 was performed using QT-PCR and immunohistochemistry.Results(1)Decidual DSC QT-PCR results: In the experimental results,T3 concentration of different decidual cells of VEGF-A,Ang-1 and Ang-2 secretion has a significant impact.The levels of 1 nMol/L,10 nMol/L and 100 nMol/L for VEGF-A secretion were increased by 1.063,4.014 and 1.080 times,respectively,for the mRNA of carbon-adsorbed serum cells.-1 secreted,respectively,increased by 3.445 times,9.151 times and 7.246 times;for Ang-2 secretion,respectively,increased by 1.080 times,3.966 times and 11.393 times.There was significant difference in mRNA content between groups.(2)Immunohistochemical analysis of decidual membrane: VEGF-A,Ang-1 and Ang-2 were observed in the cytoplasm of decidual DSC cells in different concentrations of T3,and cytoplasm was positive.Among them,the concentration of VEGF increased,VEGF-A,Ang-1 and Ang-2 expression intensity lower concentration T3,the intracellular positive staining.(3)Results of CTB QT-PCR analysis: In the experimental results,T3 concentration had different effeCTB on the secretion of VEGF-A,Ang-1 and Ang-2 in villous cells.The levels of 1 nMol/L,10 nMol/L and 100 nMol/L for VEGF-A secretion were increased by 1.572 fold,6.998 and 1.239 times,respectively,for the mRNA of mRNA in carbon-adsorbed serum cells.-1 secreted,respectively,increased by 7.503 times,20.791 times and 7.571 times;for Ang-2 secretion,respectively,increased by 4.376 times,12.126 times and 9.101 times.There was significant difference in mRNA content between groups.(4)Analysis of villus CTB results:VEGF-A,Ang-1 and Ang-2 were secreted in cytoplasm of villus CTB cells treated with different concentrations of T3,and cytoplasm was positive.Among them,the concentration of VEGF increased,VEGF-A,Ang-1 and Ang-2 expression intensity lower concentration T3,the intracellular positive staining.Conclusion1 thyroxine on decidual DSC cells and villous CTB cells have an effect on the secretion of vascular growth factor.2 thyroxine can increase the secretion of vascular growth factor VEGF-A,Ang-1 and Ang-2.3.The secretion of VEGF-A,Ang-1 and Ang-2 in the decidua DSC cells and villi CTB cell vascular growth factor is dependent on the change of thyroxine concentration.4.Hypothyroidism induced abortion may be associated with abnormal secretion of VEGF-A,Ang-1 and Ang-2 in the decidual cells and villi CTB cells.