The Expression Of The Long Noncoding RNA CTA-941F9.9 In Colorectal Cancer

Objective: Colorectal cancer(CRC)is the second most commonly diagnosed cancer in women and third in men.In 2012,an estimated 693,900 deaths due to CRC occurred worldwide,accounting for 8% of all cancer deaths.Although medical technologies and treatment strategies have made significant progress,60% of colorectal cancer patients are still diagnosed at advanced stages,and only 5% patients with distant metastasis had the outcome for more than five years.Long noncoding RNAs(lnc RNAs)are transcript species with more than 200 nucleotides and without the capacity to code proteins.Over the past few years,growing evidence has suggested that lnc RNAs may be associated with the progression of numerous diseases.Long noncoding RNAs(lnc RNAs)are involved in gene dysregulation in many types of cancer.This study aimed to investigate the expression levels of lnc RNA CTA-941F9.9 in colorectal cancer tissues and matched normal adjacent tissues(NATs).Moreover,we discussed whether this molecule could be a predictive biomarker in colorectal cancer.Methods: We collected 133 tissue samples from patients who received radical resection at the First Hospital of China Medical University in 2010 or 2016.Colorectal cancer was histopathologically confirmed in all patients.Pair-matched tissues were obtained from an area distant more than five centimeters from the tumor.All samples were snap-frozen in liquid nitrogen and stored at –80°C until use.No patients received preoperative chemotherapy or radiotherapy before resection.Total RNA was extracted from colorectal cancer tissues and NATs of 133 patients.Quantitative PCR was performed to quantify the expression levels of CTA-941F9.9 in these samples.The association between the expression levels of CTA-941F9.9 and clinicopathological characteristics was analyzed.The diagnostic value of CTA-941F9.9 was also evaluated by building a receiver operating characteristic(ROC)curve.Results: Our results indicated that the expression levels of lnc RNA CTA-941F9.9 in colorectal cancer tissues were downregulated compared with NATs(P < 0.001).Furthermore,the area under ROC curve was 0.802(sensitivity,74.3%;specificity,82.9%).We found no significant correlation between CTA-941F9.9 expression levels and age(P = 0.984),sex(P = 0.804),tumor location(P = 0.967),tumor size(P = 0.112),histological grade(P = 0.570),histological classification(P = 0.808),p T stage(P = 0.942),p N stage(P = 0.818),and p TNM stage(P = 0.849).However,no significant correlation was found between the expression levels of CTA-941F9.9 and patient outcomes.Conclusions: The lnc RNA CTA-941F9.9 was significantly downregulated in colorectal cancer tissues compared with NATs and might be a novel diagnostic biomarker for colorectal cancer.