| Objective:DEPDC1(DEP domain containing 1)is a tumor-related gene found in recent years.Previous studies have shown that DEPDC1 plays an important role in the development and progression of nasopharyngeal carcinoma.In this study,DEPDC1 was used to further investigate the effect of DEPDC1 on the proliferation of NPC cells.Methods:In nasopharyngeal carcinoma cell line CNE-1,the two spliceosomes overexpressing DEPDC1-V1 and DEPDC1-V2 were transfected respectively,and transiently expressed for 48h,then CCK8,flow cytometry and pHH3 labeling assays were used to detect cell proliferation.Results:Western blot showed that transient transfection of DEPDC1-V1 and DEPDC1-V2 in CNE-1 cells could overexpress DEPDC1.CCK8 experimental results showed that,over-expression of DEPDC1-V1 and DEPDC1-V2 significantly promote CNE-1 cell proliferation.Flow cytometry results showed that compared with the control group,overexpression of DEPDC1-V1 or DEPDC1-V2 resulted in an increase of G2/M phase cells.The result of pHH3 labeling assay showed that mitosis was active after DEPDC1-V1 or DEPDC1-V2 was overexpressed,and the cell cycle progression was accelerated.Further quantitative RT-PCR analysis showed that overexpression of DEPDC1-V1 or DEPDC1-V2 led to the up-regulation of FoxMl and its downstream target genes CCNB2,PLK1 and MYBL2 in cell cycle progression.Conclusion:In this study,overexpression of two spliceosomes,DEPDC1-V1 and DEPDC1-V2,in nasopharyngeal carcinoma CNE-1 cell lines demonstrated that DEPDC1 overexpression in nasopharyngeal carcinoma cells plays an important role in promoting cell proliferation. |
