The Expression And Significance Of HIF-1??Survivin And VEGF-c In Papillary Thyroid Carcinoma

Objective:To investigate the relationship between lymph node metastasisandclinicopathologicalcharacteristicsinPTCby analyzing clinical materials.Analyze the relationship between the expression of HIF-1?,Survivin and VEGF-c and invasion and metastasis of PTC.Methods:1.We retrospectively summarized the medical records of patients with PTC admitted to Hebei Medical Hospital during the 2 years from January2015 to December 2016,and analyzed the relationship between lymph node metastasis and clinicopathological characteristics of PTC.2.There are totally 163 paraffin-embedded specimens of pathology department of Hebei Medical Hospital from January 2015 to December 2016were selected.The expression levels of HIF-1?,Survivin and VEGF-c in 163paraffin-embedded specimens were detected by immunohistochemistry.We analyzed the relationship between the targets protein expression and clinicopathological features.Western blot was used to detect the expression of HIF-1?,Survivin and VEGF-c in 30 surgery resection fresh specimens that were selected from patients hospitalized in our hospital from January 2017 to June 2017.Then we took statistically analyzed the expression correlation in different groups.Results:1.Chi-square test showed that central cervical lymph node metastasis was related to clinical stage,and lateral cervical lymph node metastasis was related to gender,age and extra-gland invasion.Multiple regression analysis showed that age,clinical stage and tumor multifocality were independent influencing factors of central cervical lymph node metastasis;gender,age and extra-gland tumor invasion were independent influencing factors of lateral cervical lymph node metastasis.2 Results of Immunohistochemistry2.1 The expression of three proteins in PTC tissues were significantly higher than those in normal group and benign lesion group,with statistical significance?P<0.001?.There was no significant difference in normal thyroid group and benign lesion group.The expression of HIF-1?in PTC tissues was correlated with pathological stage,extra-gland invasion and lymph node metastasis.Logistic multiple regression showed that age,lymph node metastasis and extra-gland invasion were independent factors affecting HIF-1?expression.The expression of Survivin in PTC tissues was associated with extra-gland invasion and lymph node metastasis.Logistic multiple regression showed that lymph node metastasis and extra-gland invasion were independent factors affecting Survivin expression.The expression of VEGF-c in PTC tissues was correlated with pathological stage,tumor multifocality,extra-gland invasion and lymph node metastasis.Logistic multiple regression showed that age,lymph node metastasis and extra-gland invasion were the independent factors affecting VEGF-c expression.2.2 There was a positive correlation between the high expression rates of any two proteins in PTC tissues,Spearman correlation coefficients were r_s=0.284?H-S?,r_s=0.495?V-S?,r_s=0.343?H-V?,respectively,P<0.05.3 Results of Western blot3.1 The expression of HIF-1?,Survivin and VEGF-c in PTC tissues was significantly higher than that in benign lesion group,the expression level of target protein in PTC patients with lymph node metastasis was significantly higher than those PTC patients without lymph node metastasis?P<0.001?.3.2 The expression of HIF-1?,Survivin and VEGF-c in thyroid tissues was positively correlated.The Pearson correlation coefficients were r_H-S=0.989,r_H-V=0.972,r_V-S=0.960,respectively,P<0.001.Conclusions:1.Age<45 years old,higher clinical stage,multifocal tumors are independent risk factors for central cervical lymph node metastasis in patients with PTC;young male?<45 years old?patients with PTC are more likely to have lateral cervical lymph node metastases.2.The high expressions of HIF-1?,Survivin and VEGF-c enhance the ability of metastasis and invasion in PTC.3.Combined detection of HIF-1?,Survivin and VEGF-c could assist surgeon to perform better treatment,make more accurate early diagnosis and assess the risk of metastasis and recurrence precisely.