Prepartion Of Prionace Glauca Type ? Collagen/Chitosan Based Seawater Proof Nanomembranes For Wound Healing

Skin is the largest organ of human body.It plays a vital role in protecting the human body and maintaining the normal function of the human body.The skin interfaces with the environment and is the first line of defense from external factors.In life,the skin is vulnerable to internal or external injuries,such as cuts,bites,scratches,burns,and some skin diseases,such as refractory bedsore,chronic ulcers.Therefore,it is an important problem to find a suitable wound dressing,of which there are passive wound dressings,interactive wound dressings,and biological wound dressings.The biological wound dressing has a lot of advantages compared with the others.We chosen Prionace glauca cartilage type II collagen(CII)and chitosan as the main raw materials,and screened low toxic or even non-toxic solvent.The composite wound dressings were prepared by electrospinning,then the properties of the materials were analyzed.The CII and chitosan were dissolved in different solvent systems,respectively.Under suitable spinning conditions,a relatively low toxic and inexpensive solvent system for electrospinning were screened.In the experiment,it was also found that the solution had high viscosity when the chitosan was dissolved in the solvent.It will impede the extension of the fiber in the Taylor cone during the electrospinning process,so it is necessary to deal with the chitosan to reduce the viscosity of the solution.In the preliminary experiment,80% of the acetic acid solution was selected as the solvent system and the solution concentration was 0.07 g/m L The composite nanofibers were successfully prepared by electrospun technology.In order to explore the best process of electrospinning,the single factor experiment was used to determine the center point of the process parameters.The optimal process parameters are obtained by the response surface method,and the relevant parameters were set according to the actual situation:80% acetic acid/water,The blended solution concentration is 0.07 g/m L(CII:chitosan:PEO=1:5:1)applied voltage(20 k V),nozzle-collector distance(16.6 cm)and feed speed(0.70 m L/h),the minimum mean diameter of fibers was around 230.34 nm.Although the nanofiber composite membrane has good microscopic morphology,there are a large number of hydrophilic groups in the material,which makes the waterproof property of the material worse.Therefore,it is necessary to study the modification of the material.The waterproof properties of nanofiber materials are improved by using 25% glutaraldehyde water vapor to cross link nanofiber composite membranes in different time.Optimum crosslinking time was determined by the simulation of seawater immersion,tensile tests and contact angle experiments.The physical and chemical properties of cross-linked nanofiber was investigated by Fourier-transform infrared spectra(FTIR),and thermal analysis.We chose optimum crosslinking time 24 h according to the experimental result.Biocompatibility is an essential criterion for the study and final application of biomaterials.In the process of actual use,the reaction occurs when the material is interacting with the body.Preliminary explores the biocompatibility of the material from the cell proliferation.BMSC_s and L929 were planted on the composite membrane.The results showed that the cells could adhere and growth on the material.The effect of the material on the proliferation of different cells is different.It has a better effect on L929.