| Objective:We aim to investigate the expression of elongation of very long chain fatty acids protein 6(ELOVL6)in high-grade serous ovarian carcinoma(HSOC),and explore the correlation between its expression and clinical prognosis in these patients.Methods:The expression of ELOVL6 at mRNA and protein levels were detected by RT-PCR and immune histochemistry method respectively in 12 normal ovarian tissues and 172 HSOC from primary tumor site,fourty of which had paired peritoneal metastatic tissues.Results:Compared with normal ovarian tissue,the level of ELOVL6 mRNA was significantly lower in HSOC(p<0.05).The expression of ELOVL6 was lower in G3 than that in G1/2(p<0.05).There was no obvious difference between primary and peritoneal metastatic sites in HSOC.It was shown by immunostaining assay that ELOVL6 protein localized in cytoplasm of ovarian cancer cell.ELOVL6 expression was observed in all normal ovarian tissue,70%of G1/G2 and 49.0%of G3 HSOC(p<0.05).ELOVL6 expression in drug-resistant group were significantly lower than that of non-resistant group(39.0%vs.65.0%,p<0.01),The median disease-free survival was 41 months in the ELOVL6-positive group and 39 months in ELOVL6-negative group(p>0.05).The total median survival was 52 months in ELOVL6-positive group and 44 months in ELOVL6-negative group(p>0.05).Conclusion:Low expression of ELOVL6 may correlate with the poor differentiation and drug resistance in HSOC. |
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