| Objective: The aim of this study is to investigate the effect of ?-3 polyunsaturated fatty acids(?-3 PUFAs) in inflammation of obstructive jaundice(OJ)rat model and its clinical application.We hope to support the experimental and clinical data for the general application of ?-3 PUFAs.Methods: 72 healthy female Wistar rats were randomly divided into 4 groups: OJ + ?-3 group(n = 18),OJ + NS group(n = 18),SO + ?-3 group(n = 18),SO+ NS group(n = 18).The biliary duct was ligated in OJ+?-3 group and OJ+NS group to establish the model of obstructive jaundice.SO+?-3 group and SO+NS group had open abdominal surgery but did not ligate bile duct.OJ+?-3 group and SO+?-3 group were given ?-3 fish fat emulsion via intravenous injection(5ml·kg-1·d-1)after operation.OJ + NS group and SO + NS group rats were given the same volume of saline via tail vein injection.At,6 experimental rats were executed at each time point(2d,5d and 9d).Enzyme-linked immunosorbent assay(ELISA)wasapplied to detect the expressions of Lipopolysaccharide binding protein(LBP),Tumor necrosis factor-?(TNF-?),Interleukin 6(IL-6) and Toll-like receptor 4(TLR4) in obstructive jaundice rats serum;Sixty patients with obstructive jaundice were categorized as parenteral nutrition group(n = 30,group A)and parenteral nutrition +?-3 polyunsaturated fatty acids group(n = 30,group B).Two days after the obstructive jaundice was removed by different methods,group A received conventional nutritional support for 5 days,while group B received ?-3 polyunsaturated fatty acid supplementation and conventional nutritional support for 5 days.We observed the changes of serum enzymes of liver and bilirubin index in each group.Results: 1.After modeling,we obtained the IVC blood at 2d,5d and 9d,the data indicated that :(1)At 2d,compared with OJ+NS group,the levels of TNF-? in OJ+ ?-3 group were decreased,and the difference was statistically significant(P<0.05).At 9d,compared with OJ+NS group,the levels of TNF-? in OJ+ ?-3 group were decreased,and the difference was statistically significant(P<0.05).(2)At 2d,5d and 9d,the LBP levels of OJ+?-3 group were lower than those of OJ+NS group,and the difference was statistically significant(P<0.05).(3)At 2d,5d and 9d,the levels of TLR4 in the OJ+?-3 group were lower than those in OJ+NS group,and the difference was statistically significant(P < 0.05).(4)At 2d,5d and 9d,the IL-6 levels of OJ+?-3 group was lower than those of OJ+NS group,and the difference was statistically significant(P<0.05).2.The samples of blood were obtained at preoperative 3d,postoperative 2d and 8d respectively in the patients with clinical obstructive jaundice,and the data indicated that:(1)ALT?AST?ALP and GGT: compared with preoperative 3d,the above indexes of two groups at postoperative 8d and postoperative 2d were reduced,and the difference was statistically significant(P < 0.05).At postoperative 8d,the index of group B were lower compared with group A,,and the difference was statistically significant(P < 0.05).(2)TBIL and DBIL: compared with preoperative 3d,the indexes of two groups at postoperative 8d and postoperative 2d were reduced,and the difference was statistically significant(P < 0.05).At postoperative 8d,compared with group A,the indexes of group B were lower,and the difference was statistically significant(P < 0.05).Conclusion: 1.?-3 polyunsaturated fatty acids could decrease the expression of inflammatory cytokines in OJ model rats,and further improve the inflammatory state of obstructive jaundice rats and protect the organisms.2.In the patients with obstructive jaundice,the addition of ?-3 polyunsaturated fatty acids in the external nutrition could improve the liver function and decrease the bilirubin index,thus improving the nutritional status and Accelerating clinical recovery. |
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