Pharmaceutical Studies On Compound Bee Pollen Freckle-removing Effervescent Granules

Objective: Study on the rape bee pollen wall broken process optimization and the antioxidation and tyrosinase inhibition ability of bee pollen were measured.In order to enhance the beauty effect of bee pollen,the combination of aloe and licorice was selected.Select the best compatibility prescription and reveal the compatibility prescription rule to prepare the effervescent granules for reproductive treatment of Melasma.To study the preparation process and quality standard of this effervescent granules.Methods:Optimization of process parameters of extraction antioxidants from Aloe and Licorice.(1)Study on the rape bee pollen wall broken process optimization by orthogonal experiment.(2)The antioxidation and tyrosinase inhibition ability of rape bee pollen were determined.(3)The extraction method of maximum concentration of effective parts was determined by anti-oxidation and tyrosinase inhibition experiments,and the extraction process was optimized.(4)Select the best compatibility prescription and reveal the compatibility prescription rule by the antioxidant and tyrosinase experiment.(5)The mouth feeling and effervesce time were taken as index to select appropriate pharmaceutical excipients,get the best formulation and optimize the preparation of effervescent granules.(6)According to the requirements of granules in the 2015 edition of Chinese pharmacopoeia,the quality inspection of the shape,particle size,water and other indicators of this particle was carried out.Results:(1)The optimum process of the rape bee pollen wall broken is as follows.The filling rate of bee pollen was 30%,the water content was15%,the bee pollen wall broken time was 30 min.The wall fraction of bee pollen reached 99.97%.(2)The pollen of rape bee pollen after the broken wall has antioxidant capacity and tyrosinase inhibition ability,and was related to the concentration.(3)The optimum extraction process of aloe effective parts was as follows:Aloe was extracted for 1 times,the amount of water was 60.Every extraction time was 120 min and the temperature of extraction was 70℃.The optimum extraction process of Licorice effective parts are as follows:Licorice was extracted for 2 times,the amount of 70% ethanol was50.Every extraction time was 120 min.(4)Select the best compatibility prescription and reveal the compatibility prescription rule by the antioxidant and Tyrosinase experiment,the best compatibility prescription was Bee pollen:Aloe:Licorice=5:1.4:1.7.(5)The optimum formulation of Bee pollen beauty effervescent granules is as follow:diluent for starch and dextrin(ratio of 3:2),the dosage was25%;Adhesive is 70% ethanol solution,effervescent agent for citric acid and bicarbonate(ratio of 1.0:1.2),the dosage was 7.3%;For sweet taste masking agent dosage(0.25%).To quality judgment,the Bee pollen beauty effervescent granules made by various quality indexes accord with regulation,quality is better.(6)The Bee pollen beauty effervescent granules quality inspection of the indicators are in line with the "Chinese pharmacopoeia" 2015 version of the particles under request.By UV method to establish the quality standard of this granules.According to the results of several sample,made the product of total flavonoids content,the quantitative analysis showed that the content of total flavonoids should not be less than 7.7%.Conclusion:The in vivo antioxidants experiment showed that the Aloe and Licorice can improve the antioxidant and whitening effect of rape bee pollen,the treatment of Melasma is helpful for adjuvant therapy.The preparation process of The Bee pollen beauty effervescent granules agent is stable.