Expression Differences Based On BNDF,Study On The Correlation Between The Deficiency Of Marrow-sea And The Nerve Function Defect Of The Stroke

Objective: To explore the correlation between the deficiency of marrow-sea and the nerve function defect in the ischemic stroke,and to investigate the influence of the deficiency of marrow-sea on the nerve function defect through the animal experiment,and provide strong experimental support for the treatment of ischemic stroke from marrow therapy.Methods: According to the principle of randomised control,the clinical study collected 30 patients with ischemic stroke in recovery period of wind phlegm and blood stasis syndrome and patients with the deficiency of marrow-sea(referred to as marrow-sea deficient group)and 30 patients with ischemic stroke in recovery period of wind phlegm and blood stasis syndrome and non marrow-sea deficiency patients(referred to as non marrow-sea deficient group),a total of 60 cases of patients.All patients were collected for basic information,followed by clinical NIHSS,MMSE and ADL scale test.Meanwhile,serum of patients was collected,and BDNF expression was analyzed by ELISA.The general data,NIHSS,MMSE,ADL scale test results and BDNF expression of two groups were summarized,and the difference of neurological deficit between the two groups was statistically analyzed by SPSS20.0.Experimental study on a rat model of middle cerebral artery occlusion(MCAO)model,rats were divided into a sham treatment group(ST),a model control group(MC)and a Fujian tablet treatment group(FC),6 rats of each group.The expression of BDNF protein at the cervical spinal cord of rats was detected by Western blot,and the expression of BDNF m RNA in the cervical spinal cord of rats was detected by fluorescence quantitative PCR.The motor function recovery of rats was evaluated by beam walking test(BWT),to observe the effect of the Fujian tablet on the expression of BDNF protein and BDNF m RNA expression and its motor function in the cervical spinal cord of rats,and to demonstrate the effect of the deficiency of marrow-sea on nerve function.Results: The clinical study showed that there was no statistically significant difference in age,sex and educational level between the marrow-sea deficient group and the non marrow-sea deficient group.In this study,the NIHSS,MMSE and ADL scores of the patients with the marrow-sea deficient group were significantly different than those in the non marrow-sea deficient group(P<0.05).The expression of BDNF in serum was lower than that in the non marrow-sea deficient group group(P<0.05),while serum BDNF was negatively correlated with NIHSS score,and positively correlated with MMSE and ADL score.The experimental results showed that 2 weeks after operation,the gene expression and protein expression of BDNF in the cervical spinal cord tissue of the rats in the model control group were higher than those in the sham treatment group(P<0.01,compared with the sham treatment group),while the Fujian tablet treatment group increased more significantly(P<0.01,compared with the model control group).In addition,the score of the Fujian tablet treatment group and the model control group increased at the end of the 2 week after the operation,and the score of the Fujian tablet treatment group was higher than that of the model control group(P<0.01)at the end of the 2 week.Conclusion: The deficiency of marrow-sea has an effect on the nerve function defect after ischemic stroke.At the same time,the experimental study shows that the method of tonifying essence to produce marrow can effectively increase the expression of BDNF protein and BDNF m RNA in the cervical spinal cord of MCAO rats.It is suggested that the method of tonifying essence to produce marrow can further promote the recovery of nerve function after ischemic stroke.On the contrary,the deficiency of marrow-sea can further aggravate the degree of neural function defect.