| Objective:To study the effects and mechanism of Apium graveolens L.root in Xinjiang and main components on lipid metabolism in L02 cells and HepG2 cells in vitro fat accumulation model.Methods:1、A model of hepatic steatosis in the L02 cells and HepG2 cells was induced by free fatty acids（FFA）,and the in vitro fat accumulation model of the two cells was established;The oil red O staining and biochemical parameters were used to verify whether the model was successfully established;The toxicity of the drug to the cells was determined by CCK-8 kits,and the high,medium and low concentrations in the safe range of the drug were screened.2、This experiment set blank（Con）group,model（FFA）group,fenofibrate（FF）group,apigenin（AGN）group,apiin（AIN）group,extract of Apium graveolens L.root（AGRE）group.The content of adipokines and inflammatory factors was determined by enzyme-linked immunosorbent assay（Elisa）;The expressions of ADPN mRNA,AMPK mRNA and SREBP-1c mRNA were detected by qRT-PCR;P-AMPKα,P-ACC,ACC and SREBP-1C proteins were detected by Western-blot technique.Results:1、A model of hepatic steatosis in the L02cells and HepG2 cells was successfully established.The IC500 of AGN,AIN,3?-methoxy apiin（3?-MI）and AGRE on L02 cells were 49.90,541.94,224.36,896.00μg/mL,respectively;The IC500 of HepG2 cells was 64.52,430.23,302.68,and 961.00μg/mL.The levels of TG and TC in the cells were effectively reduced by AGN,AIN and AGRE（P<0.05）.2、AGRE,AGN,AIN and FF significantly decreased the levels of IL-6 and TNF-αcompared with the FFA group,（P<0.05）,and the expression of adiponectin（ADPN）secreted protein in FF group increased significantly.AGN group,AIN group and AGRE group increased the expression of ADPN mRNA and AMPK mRNA in a dose-dependent manner,and decreased the expression of SREBP-1c mRNA.In addition,the expression of P-AMPKαand P-ACC protein was also significantly increased,and the expression of SREBP-1C and ACC protein was decreased.Conclusion:AGRE has a reliable lipid-lowering and liver-protecting effect.Its mechanism may be through the regulation of AMPK/SREBP-1c signaling pathway in lipid metabolism.This study can provide experimental evidence for the development of drugs for the prevention and treatment of diseases related to lipid metabolism disorders such as non-alcohol fatty liver disease（NAFLD）. |
