In Vitro Effects Of DKK1 In Dental Follicle Cells And Its Mechanism Of Osteogenic Differentiation

Objective: To study the regulating protein of Wnt/beta-catenin signaling pathway,DKK1,inhibitor can be expressed in the dental follicle cells and after osteogenesis induction as well as the specific expression mechanism of DKK1,to explore the relationship between the formation of mature osteoblasts and eruption of the teeth.Methods: The dental follicle cells were primarily cultured by digestion method and purified.The cells were divided into 2 groups: the control group was cultured in α-MEM medium,and the experimental group was cultured in α-MEM medium supplemented with osteogenesis induction.With increased of time after intervention two groups of cells were identified of bone formation using ALP staining and immunocytochemistry.Western-blotting was used to evaluate the lever of cellular DKK1、β-catenin and Runx2 protein.Results: Compared with control group,dental follicle cells ALP staining is positive,and the factors of DKK1 expression of experimental group was significantly lower.The Western-blot results showed that the expression of DKK1 were significantly lower than that of the control group,but the osteogenic factor Runx2 and β-catenin protein expression was enhanced in the experimental group over time.Conclusion: The expression of inhibitor DKK1,the key regulator of Wnt pathway,is affected by osteogenesis induced differentiation in the dental follicle cells and reflect its inhibitory characteristics.To suggests the molecular basis of the formation mechanism for the development of Maxillary and mandible and eruption of the teeth.