Study On The SPD-1/sPD-L1 And Th1/Th2 And T17/Treg Related Cytokine In Patients With Primary Immune Thrombocytopenia

Objective: In recent years,with the deepening of the research on the pathogenesis of Primary immune thrombocytopenia(ITP),it was found that the T cellular immunity played a role in the pathogenesis of ITP.PD-1/PD-L1 negative signaling pathway can inhibits the activation of T cells.sPD-1/sPD-L1 is the soluble form of PD-1/PD-L1.The present study is To investigate the expression of sPD-1/sPD-L1 and its balance relationship with Th1/Th2,Th17/Tregin in ITP.Methods: A total of 35 patients with initial diagnosis of ITP and 20 healthy subjects were included in the present study.Peripheral blood was collected from ITP patients and healthy subjects.The levels of sPD-1,sPD-L1,IFN-γ,IL-4,IL-17 and TGF-β were detected in patients and healthy subjects by enzyme-linked immuno sorbent assay(ELISA).Results:(1)Compared with the normal control group,the level of sPD-1,IFN-γ and IL-17 in ITP patients were increased,and IL-4 and TGF-β were decreased,and no significant change in sPD-L1 level.(2)The correlation analysis showed that sPD-1 was positively correlated with IFN-γ(r=0.516,P=0.006),negatively correlated with platelet count(r=-0.562,P=0.002).The platelet count was negatively correlated with IFN-γ(r=-0.531,P=0.004).There was no correlation between sPD-1 and IL-4,IL-17 and TGF-β(P>0.05).Conclusion: sPD-1 may inhibit the PD-1/PD-L1 negative regulation pathway,lead to the imbalance of T cells subgroups,affect the immune function of T cell and playing an important role in ITP disease.