| Objective: Ischemia-reperfusion injury is an important physiological and pathological reaction in clinical surgery and medical treatment that affects the therapeutic effect and prognosis.Especially in the important organs in the cause of various diseases in the conditions leading to inadequate blood perfusion or complete blocking,after treatment,the cause was removed,the inflammatory reaction caused by the blood perfusion of tissues and organs will further lead to the injury and apoptosis of cells.At present,most studies have confirmed that hmgb-1 protein is an important inflammatory factor involved in such inflammatory response damage,however,studies on the relationship between its expression at the pathological level and the inflammatory response to renal ischemia reperfusion injury are relatively lacking.In this study,a kidney ischemia reperfusion injury model was designed in SD rats to explore the relationship between the expression of HMGB-1 protein and the inflammatory response to renal ischemia reperfusion injury.Changes of HMGB-1 protein expression level and degree of renal injury in different treatment methods and ischemia time.To provide an optimal ischemic time for the treatment of block renal blood flow at the pathological level,and provide the theoretical basis for the prevention of ischemia reperfusion injury and be the guidance of treatment.Methods : In this study,healthy SD rats were divided into Sham group(Sham group),ischemia group(group I),and ischemia-reperfusion group(IR group),and the time control of ischemia was conducted for 5min,15 min,30min,and 40 min,after the right kidney was pretreated.(1)Sham group rats were only treated with nothing after anesthesia and the operation of finding the renal pedicle was performed without blocking the renal blood flow.Antibiotics were given after waiting for the corresponding time,and sutured the wound,placed it in a separate cage for observation.After 24 hours,we anesthetized it again to obtain kidney specimens and blood samples.(2)we found and blocked the renal artery after anesthesia in I group,and renal and blood specimens were obtained directly after waiting for the corresponding time.(3)we found and blocked the renal artery after anesthesia in IR group,and the blocking was removed to restore blood after waiting for the corresponding time,and sutured the wound,placed it in a separate cage for observation.After 24 hours,we anesthetized it again to obtain kidney specimens and blood samples?(4)The kidney specimens saved in 4% paraformaldehyde fluid,for testing HE dyed,Tunel cell apoptosis,HMGB-1 expression level,blood specimens preserved in the 4 ?for serum creatinine testing.(5)Finally,use SPSS 22.0 software to analyze the data.The experimental results were expressed as "X±SD",and the experimental data were processed by multivariate analysis of variance in statistics.P<0.05 meant the difference was statistically significant.Results:(1)Sham group and treatment group: when the ischemia time was the same,there was no significant difference in HMGB-1 expression level between Sham group and treatment group at 5min and 15 min of ischemia time(P=0.146,P=0.216,P>0.05),and there was significant difference at 30 min and 40 min of ischemia time(P <0.001);In the percentage of Tunel cells apoptosis,there were statistically significant differences between the Sham group and the I group at 15,30 and 40 minutes after ischemia(P=0.029,P=0.017,P<0.001,P<0.05),and significant differences between the Sham group and the IR group at 30 and 40 minutes after ischemia(P=0.041,P <0.001,P <0.05);In the value of serum creatinine,there were significant differences between Sham group and I group at 30 min and 40 min of ischemia(P=0.008,P<0.001,both P values were >0.05),and between Sham group and the IR group were significantly different at 40 min after ischemia(P<0.001).(2)comparison between the two treatment groups: when the ischemia time was the same,HMGB-1 expression level in the I group was significantly different from that in the IR group(P<0.001 and P=0.014<0.05);In the percentage of Tunel cells apoptosis,there was a significant difference between the two treatment groups(P <0.05).After 5min,15 min,and 30 min of ischemia,the apoptosis level in the I group was higher than that in the IR group.After 40 min of ischemia,the apoptosis level in the IR group was significantly higher than that in the I group;In the value of serum creatinine,when the ischemic time was 30 min and 40 min,the difference between the two treatment groups was statistically significant(P<0.001 and P=0.001).(3)comparison within I group: under different ischemic time,the HMGB-1 expression level at 40 min of ischemia was significantly different from that at 5min,15 min and 30 min of ischemia(P <0.001);In the percentage of Tunel cells apoptosis,the value significantly higher when the ischemia time was 40 min than when the ischemia time was 5min,15 min and 30min(all P values were <0.001);In the value of serum creatinine,there were significantly higher at 40 min after ischemia than at 5min,15 min,and 30 min after ischemia(all P <0.001).(4)comparison within IR group:under different ischemic time,the differences in HMGB-1 expression level,percentage of Tunel cells apoptosis and value of serum creatinine were the same as those in the I group.The expression level at 40 min ischemic time was significantly higher than that at 5min,15 min and 30 min ischemic time(all P <0.05).Conclusion:(1)HMGB-1 is involved in the inflammatory response of renal ischemia reperfusion injury,and its expression is positively correlated with the severity of the inflammatory response of renal ischemia-reperfusion injury and the severity of renal injury.(2)Reperfusion injury at 30 min and 40 min after ischemia was significantly higher than that at 5min and 15 min after ischemia.(3)The renal injury caused by simply blocking renal blood flow for 30 min and 40 min was significantly higher than that caused by ischemia for 5min and 15 min.(4)The damage caused by simple ischemia to the kidney is higher than that caused by ischemia-reperfusion after 24 h,which may be related to the gradual recovery of renal function after 24 h. |
PDF Full Text Request