| Objective:To observe the effect of Chinese herbal compound granules on learning and memory ability of D-galactose-induced aging model mice,and to detect differential metabolism in urine of aging model mice by nuclear magnetic resonance spectroscopy(~1H-NMR)based metabolomics study.And look for the metabolic pathways of traditional Chinese medicine compound granules to prevent aging.Methods:Four weeks-old mice were randomly divided into normal control group,aging model group,Vitamin E positive control group(0.2g/kg),low,midium and high dose group of Chinese medicine Compound granule(2.0g/kg?4.0g/kg?8.0g/kg),and D-galactose-induced(100mg/kg)mice aging model The Chinese medicine compound granules were used for drug intervention.After 8 weeks of administration,the urine of each group of mice was collected within 24 hours after the last administration;the place navigation test and probe trail testing were performed by the mouse Morris water maze.Experiments were performed to observe the changes of learning and memory ability of mice in each group.Urine samples from all groups were analyzed by ~1H-NMR technology and OPLS-DA,PLS-DA method was conducted to distinguish the metabolic phenotypes and analysis which metabolic pathway.Results:Morris water maze test results showed that compared with the blank group,the number of escape platform entry times and effective area entry times of the aging model group were significantly reduced,and the effective area exercise time and exercise distance were significantly shortened,the difference was statistically significant,(P<0.05).Compared with the aging model group,the number of escape platforms in the blank control group,vitamin E-administered group,Chinese medicine compound granule low-dose group and middle-dose group increased significantly,the difference was statistically significant,(P<0.05);high-dose group The number of escape platforms in mice increased significantly,and the difference was statistically significant(P<0.01).Compared with the aging model group,the effective regional exercise time of the blank control group and the vit.E administration group increased significantly,and the difference was statistically significant(P<0.05).The active area exercise time of the high-dose group significantly increased.The difference was statistically significant,(P<0.01).Compared with the aging model group,the effective area movement distance of the blank control group was significantly increased,the difference was statistically significant,(P<0.05);the vit.E administration group,the medium dose group and the high dose group mice effective area The exercise distance increased significantly,and the difference was statistically significant(P<0.01).Compared with the aging model group,the effective area of the blank control group,the low-dose group and the medium-dose group increased significantly,the difference was statistically significant,(P<0.05);vit.E administration group and high dose group The number of effective area entries in mice increased significantly,and the difference was statistically significant(P<0.05).The step-down test:the results showed that compared with the blank control group,the latency of the first day and the second day of the aging model group increased significantly,and the number of reaction errors increased significantly,the difference was statistically significant(P<0.01).Compared with the aging model group,the latency of the first day of the blank control group,the vit.E administration group,the traditional Chinese medicine compound granules and the high dose group were significantly shortened,the difference was statistically significant,(P<0.01);low dose group The latency of the first day of the mice was significantly shortened,and the difference was statistically significant(P<0.05).Compared with the aging model group,the latency of the blank control group,the vitamin E administration group,the Chinese medicine compound granule middle dose group and the high dose group mice were significantly shortened on the second day,(P<0.01).Metabonomic analysis revealed that there were 20 different metabolites in mouse urine VLDL,isoleucine,leucine,lactic acid,alanine,choline,methionine,pyruvic acid,succinic acid,?-ketoglutaric acid,citric acid,dimethylamine,trimethylamine,taurine,glycine,creatine,allantoin,urea,hippuric acid,and formic acid;And 7 metabolic pathways such as Valine?leucine and isoleucine biosynthesis,Taurine and hypotaurine metabolism,Glyoxylate and dicarboxylate metabolism,Glycine?serine and threonine metabolism,Citrate cycle(TCA cycle),Pyruvate metabolism,Cysteine and methionine metabolism.Conclusion:D-galactose-induced aging model mice have cognitive dysfunction,which is characterized by decreased learning and memory,poor spatial localization,and decreased memory.Chinese medicine compound granules can change the cognitive dysfunction of aging model mice and improve the learning and memory ability of D-galactose aging model mice.Through metabolomics test results,it was found that Chinese medicine compound granules can improve the energy metabolism,amino acid metabolism and inflammatory response pathways in aging model mice induced by D-galactose.Chinese medicine Compound granule had a certain improvement effect on D-galactose-induced aging model mice interfere with energy metabolism,amino acid metabolism and inflammatory related pathways. |
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