The Effect Of Royal Jelly On Learning And Memory Ability And Anti-aging Of Aging Rats

This paper takes adult SD rats as experimental material.The sub-acutely aging rats were made by injecting 2.5% D-galactose solution into the rats'abdominal cavity continuously,last 45 days. After it, administered intragastrically with different concentrations of royal jelly for 90 days. The learning and memory ability of rats in each group were detected with Morris water maze.The monoamine transmitter contents such as norepinephrine and dopamine in the brain were detected with Fluorescence spectrophotometer in order to observe the effect of royal jelly on learning and memory ability of aging rats .Then detect the variation of the activity of SOD and the content of MDA in each rats, blood serum and lung and spleen, in order to observe the effect of royal jelly on anti-aging activity.The results show that the learning and memory ability of aging rats dropped significantly compared with normal rats. The average latent period of the model group extended distinctly, the latency prolonged from 15.52s to 21.62s. In the probe test, the number of traversing originally platform dropped obviously, the number dropped from 7.5 to 4.25 (P﹤0.05) ,the types of swimming track of the model group changed remarkably. The NE, DA contents in the brain of aging rats decreased significantly,the activity of SOD also decreased significantly, the content of MDA rising markedly(P﹤0.05). we can indicate that the sub-acutely aging rats was successfully manufactured.While the learning and memory ability of royal jelly rats improved significantly compared with aging rats, The average latent period shortened obviously from 21.62s to 14.34s(P﹤0.01), In the probe test, the number of traversing originally platform improved obviously, the number improved from 4.25 to 8.22 (P﹤0.01).The NE, DA contents in the brain increased from 196.87ng/g,160.75ng/g to 314.62ng/g,251.00ng/g respectively (P﹤0.01). The activity of SOD enhanced, the contents of MDA dropped obviously in royal jelly rats, blood serum and lung and spleen.These results indicate that royal jelly can improve spatial learning and memory ability of aging rats and content of monoamine neurotransmitters, SOD activity, can reduce the MDA contents. It can inferred that royal jelly can regulate the metabolized of monoamine transmitter effectively and anti-aging, improve the learning and memory ability.