Expression Of ASPP Family In Cutaneous Malignant Melanoma And Its Clinical Significance

ObjectiveImmunohistochemical method was used to investigate the expression and mechanism of p53 apoptosis-stimulated protein gene family(ASPP)in cutaneous malignant melanoma(CMM)tissue,and to analyze its correlation with clinicopathologic features of CMM.Methods40 cases of CMM tissue.10 cases of nevus cell nevus tissue and 10 cases of normal skin tissue diagnosed by pathology were collected from January 2001 to December 2017 in our hospital.40 CMM patients were classified by Clark grading method into 13 cases grade ?.14 cases grade ?-?.13 cases grade ?-?,13 cases in situ,27 cases invasive,10 cases lymph node metastasis and 30 cases non-lymph node metastasis.The expression of ASPP1,ASPP2 and iASPP in normal skin tissue,nevus cell nevus tissue and CMM tissue was detected by immunohistochemical SP method.The correlation between ASPP1,ASPP2 and iASPP proteins and the clinicopathologic features of CMM patients,such as sex,age,Clark grade.lymph node metastasis and invasion.was analyzed.Results1?The expression of iASPP protein in normal skin(3.220±1.351).nevus cell nevus(5.160±1.720)and CMM(9.165±2.144)showed significant difference among the three groups(F=44.699.P<0.001);the expression of iASPP protein in nevus cell nevus tissue was much lower than CMM tissue and obvious higer than normal skin tissue(all P<0.05).2?The expression of ASPP1 protein in normal skin(3.100±1.860).nevus cell nevus(5.280±1.971)and CMM(8.695±2.457)showed significant difference among the three groups(F=27.820,P<0.001):the expression of ASPP1 protein in nevus cell nevus tissue was much lower than CMM tissue and obvious higer than normal skin tissue(all P<0.05).3?The expression of ASPP2 protein in normal skin(3.180±1.812),nevus cell nevus(5.140±1.886)and CMM(8.785±2.314)showed significant difference among the three groups(F=32.096,P<0.001);the expression of ASPP2 protein in nevus cell nevus tissue was much lower than CMM tissue and obvious higer than normal skin tissue(all P<0.05).4?The expression level of iASPP and ASPP2 in CMM tissue was not correlated with sex,age,Clark grade,lymph node metastasis and invasion,and there was no significant difference(P>0.05).5?The expression level of ASPP1 in CMM tissue was not correlated with sex and age of patients,and there was no significant difference(P>0.05);While The expression level of ASPP1 in CMM tissue was increased with the increase of Clark grade and lymph node metastasis,and the expression level of invasive CMM was significantly higher than that in situ CMM(P<0.05).6?Pearson correlation analysis showed that the expression levels of iASPP,ASPP1 and ASPP2 in CMM tissue were not correlated with each other(P>0.05).7?Pearson correlation analysis showed that the expression levels of iASPP,ASPP1 and ASPP2 in nevus cell nevus tissue were not correlated with each other(P>0.05).8?Pearson correlation analysis showed that the expression levels of iASPP.ASPP1 and ASPP2 in normal skin tissue were not correlated with each other(P>0.05).Conclusions1?The expression level of iASPP protein in CMM tissue was significantly higher than that in nevus cell nevus tissue and normal skin tissue.2?The expression level of ASPP1 protein in CMM tissue was significantly higher than that in nevus cell nevus tissue and normal skin tissue.3?The expression level of ASPP2 protein in CMM tissue was significantly higher than that in nevus cell nevus tissue and normal skin tissue.4?The expression levels of iASPP protein and ASPP2 protein were not correlated with Clark grade,lymph node metastasis and invasion of CMM.5?The expression level of ASPPI protein is correlated with Clark grade.lymph node metastasis and invasion of CMM.6?There was no correlation between the expression level of iASPP,ASPP1 and ASPP2 in CMM.