Effect Of Qingnao Yiyuan Decoction On Expression Of GFAP And NeuN In Rats With Cerebral Ischemia Injury

Objective: To observe the effect of Qingnao Yiyuan Decoction on the expression of GFAP and Neu N in rats with cerebral ischemia injury,and to explore the mechanism of Qingnao Yiyuan Decoction in protecting neurons from cerebral ischemic injury.Methods: A total of 112 healthy male Sprague-Dawley rats weighing 250±50g were randomly divided into 4 groups: blank group(7 rats),sham operation group(35 rats),model group(35 rats)and qingnaoyiyuan group(35 rats).The sham operation group,the model group and the Qingnao Yiyuan decoction group were further divided into 5 subgroups according to the time points of postoperative materials,1d,3d,7d,14 d and 28 d.Rats in the blank group were fed with distilled water of equal volume;In the sham-operated group,the carotid artery was inserted into a cork of about 8 mm.After normal operation,the same volume of distilled water was administered;The model group was given an equal volume of distilled water for 7 days before operation,and MCAO after anesthesia to make acute cerebral ischemia injury model.The rats were fed normally after operation and given equal volume distilled water by gavage;In theQingnao Yiyuan group,the volume of Qingnao Yiyuan Decoction was administered to the rats for 7 days before operation.MCAO was performed and the Qingnao Yiyuan Decoction was administered.The Longa 5 grade 4 method was used to score the neurological deficit symptoms after cerebral ischemic injury in rats,and the specimens were collected according to the time points of each material.After rat brain tissue was taken,the infarct volume was detected by TTC staining.TUNEL assay was used to detect the number of apoptosis of nerve cells in brain tissue.Immunohistochemical method was used to detect the changes of GFAP and Neu N positive cells in the infarct area of rats with cerebral ischemia injury.Results:(1)Neurological deficit score: Compared with the sham operation group,the neurological deficit scores of the model group and Qingnao Yiyuan Decoction group were significantly different(P<0.01);compared with the model group,at the same time The symptoms of neurological deficits in the rats of the Qingnao Yiyuan Decoction group were improved to different extents,and the difference was statistically significant(P<0.05).(2)Detection of cerebral infarction volume by TTC staining: white infarction was not seen in blank group and sham operation group,white infarction of brain tissue was observed in model group and Qingnao Yiyuan decoction group;compared with model group,Qingnaoyi The area of cerebral infarction in Yuantang group wassignificantly decreased(P<0.05).(3)TUNEL assay for apoptosis:Compared with the sham operation group,the positive expression of apoptotic cells in the model group and Qingnao Yiyuan group increased significantly at each time point(P<0.05);compared with the model group,The expression of apoptotic cells in Qingnayiyi group was significantly decreased at each time point,and the optical density of TUNEL-positive reaction was significantly decreased(P<0.05).(4)Immunohistochemical detection results: A small amount of GFAP immunoreactive cells were observed in the brain tissue of the blank group and the sham operation group,and a large number of Neu N positive cells were expressed;compared with the sham operation group,the model group and Qingnaoyi The expression of GFAP immunoreactive cells in the tuples increased significantly at the same time point,and the expression of Neu N positive cells was less,which was significantly different(P<0.05).The expression of GFAP decreased after reaching the peak at 7d,and the same time was obtained from the model group.In comparison,the expression of GFAP in Qingnao Yiyuan Decoction group was significantly decreased,and the number of Neu N positive cells was significantly increased,the difference was statistically significant(P<0.05).Conclusion: Qingnao Yiyuan Decoction has protective effects on neurons in rats with cerebral ischemia injury,and its mechanism may be related to down-regulating the expression level of GFAP and up-regulating theexpression level of Neu N.