Preparation Of Gold Nanoparticles And Imaging Studies In Ovarian Cancer Cell Lines

Background:Ovarian cancer is the second most common female genital malignancy in the world,and its mortality rate is the highest in gynecological tumors.Globally,about 200,000 people are diagnosed with ovarian cancer each year and 125,000 die from the disease.The exact etiology of ovarian cancer is still unknown,according to the literature,which may be related to environmental factors,reproductive factors,behavioral factors and genetic factors.At present,the diagnosis of ovarian cancer include symptomatic,physical examination,tumor marker,imaging,proteomics,etc.However,because of its insidious onset,early symptoms are not obvious,and the existing auxiliary examination means cannot be screened out in the early stage.Therefore,it is more important and urgent to study a kind of screening method which is harmless to the body and low cost,which can be widely used to screen early ovarian cancer.In recent years,nano-materials and nanotechnology have developed rapidly and have been widely used in many fields,providing a new opportunity for the establishment of effective cancer diagnosis and treatment.The combination of noble metal nanoparticles and light sources is particularly noticeable,and is an effective strategy for treating and diagnosing cancer.Gold nanorods(GNRs)is a rod-shaped nano materials,generally refers to the length to diameter ratio is between 2 and 25 of nanoparticles,in recent years has been used for various types of biomedical applications,and because of its unique optical properties and is widely used as a light students medical imaging method of contrast agents and solar-thermal tumor treatment.The fluorescence imaging of nanoparticles has the advantages of high strength,high detection sensitivity and high biological marker rate,and may be used for real-time imaging in surgery.Although there are still many problems to be solved,the fluorescence imaging of gold nanorods still has extensive research prospects.Objective:The novel composite nanoparticles with uniform dispersion were prepared with strong biological compatibility,and the images of ovarian cancer cells combined with gold nanorods could be observed under stable light sources.Method:The SKOV3 cell line of ovarian cancer was cultured in vitro;According to the optical principle,the crystal growth method was used to prepare gold nanorods,and the surface of the gold nano-rods was modified with anti-EGFR to make it biocompatible.Then the SKOV3 cell line imaging of ovarian cancer was observed under a stable fluorescence microscope.Result:When the gold nanoparticles connected to the anti-EGFR were added to the cells,the cells' light intensity increased under the microscope.Most tumor cells have epidermal growth factor receptors on the surface,so the gold nanoparticles connected with anti-EGFR can be specifically adsorbent on the surface of ovarian cancer cells.Under the microscope the tumor cell's nucleus is illuminated by glowing instructions that the modified gold nanorods have been attached to the anti-egfr and into the cells.Conclusion:Gold nanoparticles were prepared by crystal growth method with simple synthesis steps.The gold nanorods with different length ratios can be prepared by changing different experimental parameters.In this experiment,a large diameter ratio of gold nano-rods was synthesized,which could reduce or delay the uptake of cells,so the infiltration and retention capacity of tumor tissues was stronger.After modification,the anti-EGFR was modified to observe the ovarian cancer cell images combined with gold nanorods under a fluorescence microscope.