Surface Activity And Anti-infection Performance Research For The Application Of Dental Implants

Dental implants have become the most ideal therapeutic method for teeth injury,because of no damage to adjacent teeth,good masticatory function recovery,comfortable and beautiful,etc.The characteristics of implant surface play a vital role in initial stability and good osseointegration.Sandblasted and acid etched surface with multiple cavity structure is more conducive to cell adhesion and extension,which made it widely used.The titanium dioxide surface was achieved by PECVD before,which was superhydrophilic.Due to the adsorption of organic hydrocarbon compounds in the air,the titanium oxide surface becomes gradually hydrophobic.Hydrophobic surface of the implant isn't conductive to protein adsorption and affects the biological activity.The implant surface of titanium oxide is treated by different methods in order to keep or recover its surface biological activity.Freshly prepared oxidation titanium surface is stored in O.Olmol/L sodium hydroxide solution,0.9%solution physiologique and water,in order to avoid the air pollution of organic hydrocarbon.The results show that titanium oxide samples can keep superhydrophilic,when samples are stored in 0.01 mol/L sodium hydroxide solution and 0.9%solution physiologique directly.The oxidized titanium surface stored in 0.01lmol/L sodium hydroxide solution also maintains the same superhydrophilicity with cleaning to simulate the effects of preoperative cleansing and residual liquid in clinical practice.However,for solution physiologique storage contact angles on the titanium oxide surface rises to 9°,it's still superhydrophilic surface.The preservation of the fresh titanium oxide surface with pure water can maintain its superhydrophilicity,and spherical nanoparticles are formed on the surface,which is titanium oxide by XPS test.The average arithmetic mean deviation of samples with plasma oxidation was larger than that of SLA,but it slightly decreased after immersing in pure water.The C-H content of titanium oxide surface stored in pure water was significantly lower than that of the samples placed in air.The hydrophobic surface recovered superhydrophilicity after sputtering with Ar plasma for lmin,the parameters are:substrate bias voltage of 350V,duty ratio of 30%and gas flow of 10sccm.The superhydrophilic surface is obtained by ultrasonic cleaning with artificial saliva,O.Olmol/L NaOH solution and 0.9%physiological saline.Although the time keeping superhydrophilic by these two methods is short,it is sufficient for clinical application.The hydrophobic surface hardly recovered superhydrophilicity after rinsed vigorously with those four liquids for 30s.There was no deposition of hydroxyapatite on the surfaces of SLA and TiO,after 1 week immersion in SBF solution.After 2 weeks of immersion,a small amount of Ca and P deposits appeared on the surface of SLA and TiOx.The Ca and P deposits on TiOx surface are a little more than SLA,but the difference isn't obvious.Bovine serum albumin and fibronectin adsorption experiments showed that the protein adsorption rate of TiOx samples stored in alkaline solution for a long time is the highest;TiOx surface with alkaline rinsing for 10s following,protein adsorption rate of SLA surface is the lowest.The results of cytoskeleton staining were the same as the protein adsorption experiments.The surface oxidation of SLA and TC4 by PECVD showed good antibacterial activity.