| Objective: Testing the content and variety of antioxidant activity of different parts of Dendrobium officinale,which provided a theoretical basis for the full development and utilization of new resources.Methods:Detected by phenol sulfuric acid method of polysaccharide content,reducing sugar content was determined by DNS and flavonoid content in aluminum nitrate assay;Dendrobium officinale polysaccharide in optimal choice by comparing three trichloroacetic acid method,Sevag method,trypsin and trypsin Sevag method;monosaccharide were determined by PMP derivatization-high performance liquid chromatography(HPLC);antioxidant activity in vitro by determination of total reducing capacity using DPPH free radicals,hydroxyl radicals,superoxide anion scavenging rate.Results:Growth of different age,different harvest time and different areas in different parts of Dendrobium officinale(stem and leaf)have significant difference between the content of active ingredient,polysaccharide as an active ingredient most content of Dendrobium officinale,two years old stem was the most abundant,one year old leaves in reducing sugar content is the highest,mainly flavonoids focus on leaf;trypsin – Sevag(enzyme solution sixty minutes,extraction four times)method for removing protein optimal selection;origin,light conditions and picking time have influence on the composition of monosaccharide in stems and leaves of Dendrobium officinale,the monosaccharides in the stem are mainly mannose and glucose,the monosaccharides in leaves consist mainly of mannose,glucose,galactose and arabinose;Polysaccharides and flavonoids extracted from different parts of Dendrobium officinale showed different antioxidant activity in different antioxidant systems in vitro.The antioxidant activity of flavonoids is much higher than that of polysaccharide.Conclusions: The experimental results for better development and utilization of Dendrobium officinale resources provide a theoretical basis. |
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