Changes Of Major Nutritional Components Such As Isoflavones And Their Effects On Menopausal Syndrome During Germination Of Black Beans With Trace Elements

Objective: The primary pharmacodynamics of improving climacteric syndrome was studied by adding micronutrients to germinate and enhancing the nutritional value of black beans,and the process of adding microelements to germination,the change of nutrients before and after germination,and the addition of trace elements to black soybeans to germinate.Method: Orthogonal test method was used to determine the isoflavone extraction process using black soybean isoflavones as the index.The response surface methodology was used to determine the content of isoflavones in black soybeans as an indicator of the addition of zinc,manganese,copper,and iron to black beans.Optimization of trace element germination process;determination of isoflavone aglycones and glycosides by HPLC;determination of protein content by Kjeldahl method;determination of peptide molecular weight distribution by high performance gel filtration chromatography;colorimetric method The contents of peptides,free amino acids,polysaccharides,polyphenols,and sapogenin were determined.The contents of trace elements were determined by flame atomic absorption spectrometry and inductively coupled plasma atomic emission spectrometry.Using healthy female SD rats as experimental animals,a castration animal model was prepared to examine the effect of black bean addition of trace elements on the reproductive endocrine of ovariectomized female rats.Results: The best extraction process of isoflavones from black soybean was extraction temperature 70℃,ethanol concentration 60%,extraction time 30 min,solid-liquid ratio 1:20,and the best conditions for black bean adding microelement germination were soaking temperature 25℃,soaking time 6h,germinating temperature.25℃,germination length 12cm;through quantitative analysis,black beans add trace elements after germination,can increase the content of major nutrients such as isoflavones in black beans,of which the most significant increase in isoflavone aglycone content,therefore,select the isoflavones during germination The maximum aglycon content,ie 12 cm shoot length,is the end point of germination.At this time,the content of isoflavone aglycone was increased by 1160.00% compared with ungerminated black beans;the protein content did not change significantly,the polypeptide content increased by 33.84%,the free amino acid content increased by 1835.48%,and the total sugar content decreased.Increased sugar content led to a 68.20% reduction in polysaccharide content,a 24.00%increase in polyphenol content,a 7.01% increase in diosgenin content,a 17.82% decrease in fat content,a 12.30% decrease in phytic acid content,and a trace element result indicating that black beans were added.The contents of organic zinc,organic manganese,organic copper,and organic iron all increased after germination of the trace elements.The conversion rates were 123.29%,2.31%,69.40% and 9.03% respectively;the addition of trace elements to the ovariectomized rats was gavage.After the black soybean meal,compared with the model group,the body weight of the rats decreased,the degree of keratinization of the vaginal epithelial cells increased,the leukocyte content decreased,the FSH level decreased,and the thickness of the endometrium increased significantly.The number of bodies increased,pathological hyperplasia decreased,and inflammatory infiltration reduced.Conclusion: The addition of trace elements in black soybeans has higher nutritional value after germination,and can comprehensively improve the symptoms of climacteric syndrome in female rats to some extent.