Effects Of MicroRNA-27a-3p On Proliferation,Apoptosis And Cell Cycle Of Hepatoma Cells

Objective:To investigate the effects of miR-27a-3p on proliferation,apoptosis and cell cycle of hepatoma cells.Methods:TCGA database was used to analyze the expression of miR-27a-3p in hepatocellular carcinoma tissues and adjacent tissues,and to analyze the correlations between miR-27a-3p levels and clinicopathological features in HCC patients.Real-time polymerase chain reaction(qPCR)was used to detect differential expression levels of miR-27a-3p in normal hepatic epithelial cells(L02)and hepatoma cells(HepG 2and PLC).Using Liposome transfection method,miR-27a-3p was over-expressed in the HepG2 cells and knocked-down in the PLC cells,The cell experiment was divided into four groups : overexpression of miR-27a-3p group(miR-27a)and negative control group(miR-Con)in HepG2 cells;downregulation of miR-27a-3p group(miR-inhibitor-27a)and negative control group(miR-inhibitor-Con)in PLC cells.The expression of miR-27a-3p in these cells was confirmed by real-time qPCR after transfecting.proliferation was detected by MTT assay,Apoptosis and cell cycle was detected by flow cytometry.Results:TCGA results showed that miR-27a-3p was significantly down-expressed in the tumor tissue,and Kaplan-Meier survival analysis was performed to assess the effect of miR-27a-3p on the overall survival,high miR-27a-3p expression significantly correlated with better overall survival in HCC patients.Moreover,the level of miR-27a-3p in HCC was significantly correlated with metastasis,Race and Child-Pugh Grade,but not correlated with the T and N stage,and differentiation grade.The results of qPCR showed that the expression levels of miR-27a-3p in L02,HepG2 and PLC increased in turn,(P<0.05).MTT assay showed that,compared with miR-Con group,the cell viability in miR-27a-3p overexpression cell group wes significantly decreased(P<0.05).The apoptosis results suggested that,compared to miR-Con group,miR-27a-3p group had obiously enhancived apoptosis rate in the HepG2 overexpressed cells,(P<0.05).The cell cycle results showed that in the HepG2 overexpressed cells,the S period was lower than miR-Con Group,(P<0.05).To further verify the function of miR-27a-3p,we knocked down the expression of miR-27a-3p in PLC cells.The above experimental results were contrary to the results of HepG2 overexpressed cells(P<0.05).Conclusion:miR-27a-3p can significantly inhibit the proliferation of hepatoma cells,promote cell apoptosis,alter the distribution of cell cycle and may becomen a potential target in hepatoeellular carcinoma therapy.