| Objective: The aim of this study was to investigate the effects of dexmedetomidine with different concentration gradients on a series of biological behaviors such as proliferation,migration,cloning,cell cycle and apoptosis of HepG2 cells,and to investigate the effect of dexmedetomidine on hepatoma cells in vitro.The role of the clinical use of dexmedetomidine provides a certain reference.Methods:Human hepatoma HepG2 cells were cultured in 6-well,24-well or 96-well culture plates,and human hepatoma HepG2 cells were treated with different concentrations of dexmedetomidine,respectively,and divided into control group(group C,only added).Complete medium),dexmedetomidine1ng/ml group(D1 group),dexmedetomidine 10ng/ml group(D2 group),dexmedetomidine 100ng/ml group(D3 group),dexamethasone A total of 5groups of cells in the 1000 ng/ml group(D4 group)were cultured for 24 h,48h,72 h or 10 days.The drug was diluted with DMEM complete medium containing10% fetal bovine serum and adjusted with buffer.The pH of the medium was set to 3 replicate wells in each group as a parallel group.The effect of dexmedetomidine on the proliferation of HepG2 cells was detected by CCK-8method.The effect of dexmedetomidine on the migration ability of HepG2 cells was detected by Transwell method.The assay of dexmedetomidine on HepG2 was performed by plate cloning assay.Effect of cell cloning ability,flow cytometry to detect the effect of dexmedetomidine on cell cycle and apoptosis of HepG2.Results:After using different concentrations of dexmedetomidine to treat HepG2 cells,the CCK-8 test results showed that the absorbance values(A450values)of the wavelengths of 450 nm in the C,D1,D2,D3,and D4 groups were(1.33 ± 0.22).(1.95±0.11)(2.23±0.08)(1.5±0.13)(1.45±0.12);Transwell cell migration experiments showed that the cell migration numbers of C,D1,D2,D3,and D4 groups were(1935.50±291.75),respectively(3170.33).±542.48)(4528.67±778.51)(2185.00±648.00)(2097.67±471.46);cell cloning experiments showed that the number of cells in the C,D1,D2,D3,and D4 groups was(183.17±100.11)(657.17±218.47),respectively.(1088.33 ± 230.54)(228.33 ± 136.95)(238.33 ± 105.36),the above results indicate that the ability of cells to proliferate,migrate and clone in D1 and D2 groups is enhanced after intervention of dexmedetomidine in HepG2 cells.It indicated that dexmedetomidine can promote the biological activity of HepG2 cells in a certain extent,while the proliferation,migration and cell clone number of cells in D3 and D4 groups were not statistically different from the control group.Flow cytometry detection of cell cycle experiments showed that the percentage of cells in the G1 phase of C,D1,D2,D3,and D4 groups was(44.06±1.46)(40.58±1.61)(36.79±1.18)(43.79±1.42),respectively.(44.76±1.39),the percentage of cells staying in S phase were(8.29±0.48)(9.54±0.52)(11.81±1.02)(8.48±0.45)(8.46±0.57),and the percentage of cells staying in G2 phase were(47.64±1.70)(49.88±1.78)(51.39±1.81)(47.73±1.06)(46.78±1.57),the aboveexperimental results showed that the number of cells in the D1 and D2 groups staying in the G1 phase decreased,while staying in The number of cells in S phase and G2 phase increased,indicating that cell proliferation was more active,cell growth was more vigorous,and there was no significant difference in cell cycle between cells in D3 and D4 groups compared with the control group.Flow cytometry detection of apoptosis showed that the apoptosis rates of C,D1,D2,D3,and D4 groups were(14.01±2.18)(9.22±2.13)(8.13±2.78)(14.26±1.97)(14.77).±1.81),the above experimental results showed that the apoptotic rate in the D1 and D2 groups was significantly lower than that in the other groups,and the cell survival rate was higher,while the apoptosis rate in the D3 and D4 groups was not significantly different from the control group.Conclusion: Dexmedetomidine promotes the proliferation,cloning and migration of human hepatoma HepG2 cells at low concentrations.Flow cytometry results show that dexmedetomidine can promote faster maturation and reduce cell wilting.Death rate and a certain concentration dependence at low concentrations.However,a series of biological effects of high concentration of dexmedetomidine on hepatocellular carcinoma HepG2 cells were not statistically significant compared with the control group,indicating that dexmedetomidine has a certain concentration selectivity for the biological activity of hepatoma cells. |
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