Clinical Study Of Peripheral Blood Cytokines In Patients With Acute Spinal Cord Injury

Objective: To investigate the levels and clinical significance of interleukin-6(IL-6),brain-derived neurotrophic factor(BDNF),basic fibroblast growth factor(BFGF),neurotrophic factor 3(NT-3)and neurotrophic factor 4(NT-4).in peripheral blood after acute spinal cord injury(ASCI).Methods: Twenty-nine patients with acute spinal cord injury admitted at the Department of Spinal Osteopathia,the First Affiliated Hospital of Guangxi Medical University from October 2016 to April 2019 were enrolled,and were divided into two groups according to American Spinal Injury Association impairment scale: complete spinal cord injury(n=11)and incomplete spinal cord injury(n=18).Thirteen patients with simple spinal fracture were selected as fracture group,and Thirteen patients with avascular necrosis of the femoral head were selected as controls.The expression levels of interleukin-6,brain derived neurotrophic factor,basic fibroblast growth factor,neurotrophin-3 and neurotrophin-4 in peripheral blood of 55 patients were determined by ELISA and compared.Results: The ELISA results showed that the expression levels of interleukin-6,brain derived neurotrophic factor,basic fibroblast growth factor,neurotrophin-3 and neurotrophin-4 in peripheral blood in the spinal cord injury group were significantly higher than the fracture group and the control group(P< 0.05).The expression levels of all above cytokines in the complete spinal cord injury group were significantly higher than those in the incomplete spinal cord injury group(P < 0.05),and There was no significant difference in the expression of IL-6,BDNF,bFGF,NT3,NT4 between the fracture group and the control group(P > 0.05).Conclusions: increased expression of interleukin-6,brain derived neurotrophic factor,basic fibroblast growth factor,neurotrophin-3,neurotrophin-4 after acute spinal cord injury indicates that it may participate in the important pathophysiological process after acute spinal cord injury.It also could be used to judge the severity and prognosis of ASCI and provide reference for clinical diagnosis and treatment.