Association Of CRP Gene Polymorphisms With Susceptibility To Non-Small Cell Lung Cancer

Objectives In this study,we detected the association between CRP gene polymorphisms at position rs2794521,rs2808630 and rs1205 and the risk of non-small cell lung cancer(NSCLC)diseases,which would provides new genetic markers for assessing the risk of lung cancer and provides new clues and new ideas for the early diagnosis and prevention and treatment of lung cancer.Methods 152 patients with non-small cell lung cancer and 131 healthy volunteers were selected for the case-control study.The polymorphisms of rs2794521,rs2808630 and rs1205 loci of CRP gene was detected by SNaPshot method.The distribution frequency of genotypic and allelic genes was obtained by direct calculation.The distribution of the three loci of CRP gene in the case group and the control group was detected by ?2 test to estimate whether the distribution of the three loci in the case group and the control group was in accordance with the law of Hardy-Weinberg equilibrium or not..Odds ratio(OR)and 95% confidential interval(CI)were obtained by logistic regression analysis after correcting the related confounding factors and evaluate the association CRPgene polymorphisms and the risk of NSCLC.Finally,the haplotypes of three SNP loci were constructed by SHEsis software online,and the difference of haplotype frequency between the control group and the case group was compared.P values below the conventional level of significance(P < 0.05)were considered statistically significant.All statistical analyses were performed with SPSS software(version 17.0).Results1.There were significant differences in gender and smoking status composition between the case group and the control group(P < 0.05).The difference of age composition were not statistically different(P > 0.05).The genotype distribution of the three SNP loci in the patient group and the control group was in agreement with Hardy-Weinberg equilibrium(P > 0.05),indicating that all the samples were well represented in the population.2.We tested TT,CT and CC genotypes in rs2794521 locus,TT,CT and CC genotypes in rs2808630 locus and TT,CT and CC genotypes in rs1205 locus of CRP gene.No significant difference in the frequency distribution of each genotype and allele among the three loci was observed between the control group and the case group(P > 0.05).Logistic regression analysis was used to calculate the risk factors of sex,age and smoking history.The results showed that the genetic polymorphisms of the three loci was not associated with the risk of NSCLC.3.Gender stratification analysis showed that there was no associationbetween the polymorphisms of the three loci and the risk of NSCLC in the control group and the case group(P > 0.05),regardless male or female.When stratified by age,for those over age 50,the frequency distribution of the three genotypes of TT,CT,CC at rs1205 locus was significantly different between the control group and the case group(P < 0.05),and the genotypes at the other two sites were not significantly different.For the rs1205 locus,compared with individuals with TT genotype,individuals with CT genotype had a 2.03 times higher risk of lung cancer(OR=2.030,95%CI=1.055-3.906).When stratified analysis of smoking history,it showed that individuals with CT genotype had a2.03 times higher risk of lung cancer(OR=2.108,95%CI=1.068-4.158),compared with individuals with TT genotype,for the rs1205 locus in non-smokers.In smoking population,rs2794521 CT genotype significantly decreased the risk of lung cancer(OR=0.383,95%CI=0.153-0.962).In smoking population,rs2808630 CT genotype and C allele may be associated with lower risk of lung cancer(OR=0.348,95%CI=0.137-0.881;OR=0.405,95%CI=0.185-0.888),and rs2808630 CT genotype and C allele might be protective factors for Lung cancer.4.The haplotype analysis for rs2794521,rs2808630 and rs1205 showed no significant difference of haplotypes between the control group and the case group(P > 0.05).Conclusions1.Smoking is a risk factor for NSCLC.2.The CT genotype of rs1205 site of CRP gene may be a risk factor for lung cancer in people aged ?50 years old,and it may also be a risk factor for lung cancer in non-smokers.3.The CT genotype of rs2794521 locus of CRP gene may play a protective role in the risk of NSCLC in smokers.4.The CT genotype and C allele of CRP rs2808630 locus may play a protective role in the risk of NSCLC in smokers.