Expression Of Serum TGF-?1 In Rat Model Of Gingival Overgrowth Induced By Nifedipine And Cyclosporine A

Objective A rat model of gingival overgrowth induced by nifedipine and cyclosporine A was established and the correlation between serum TGF-?1 expression and drug-induced gingival hyperplasia was analyzed.Methods Forty male Sprague-Dawley rats aged 6 weeks were randomly divided into 4 groups: blank control group,nifedipine administration combined,cyclosporine A administration group,nifedipine + cyclosporine A combined administration group.After 42 days of dosing,all rats were treated with general anesthesia,apical(left ventricular)arterial blood was collected,and bilateral maxillary molars and periodontal tissues were isolated.The thickness of the gingival epithelium,the width and height of the connective tissue were preliminarily analyzed and measured by photographing the intraoral photos and histological measurements.The differences in the degree of gingival overgrowth were compared.The difference of serum TGF-?1 expression among each groups was detected by ELISA.Results On the 42 nd day after administration,it was observed that the gingival overgrowth was more obvious in the experimental group than in the control group,and the buccal side was heavier than the lingual side.According to the degree of gi ngival overgrowth,nifedipine + cyclosporine A combined administration group > cyclosporine A administration group > nifedipine administration group> blank control group.The expression of serum TGF-?1 was higher in the experimental group than in the control group,and the more severe the gingival hyperplasia,the higher the expression ofTGF-?1.Conclusion The combination of nifedipine and cyclosporine A can induce more severe gingival overgrowth,and the severity of drug-induced gingival overgrowth may be positively correlated with the concentration of serum TGF-?1.