Analysis Of Faeces And Tongue Coating Flora In Diarrhea-predominant Irritable Bowel Syndrome(Spleen-stomach Weakness Type)

ObjectiveTo study the characteristics and correlation of feces and tongue coating flora in patients with diarrhea-predominant irritable bowel syndrome(spleen-stomach weakness),and to investigate the food factors that induce or aggravate IBS-D patients,so as to provide new ideas for the prevention and treatment of the disease.MethodFifty-two IBS-D patients with spleen and stomach weakness were selected from the outpatient and ward of spleen and stomach disease department of Hubei Traditional Chinese Medicine Hospital(Guanggu Hospital)from July 2018 to October 2018.And they are all related to diet(such as raw cold,greasy,spicy,etc.)Illumina Miseq high throughput sequencing and bioinformatics analysis of 16 SrRNA V4-V5 gene highly variable region in tongue coating and fecal flora.To understand the characteristics and correlation of faeces and tongue coating flora in these patients,and to investigate the food factors that induce or aggravate IBS-D patients.Result1.By investigating 52 IBS-D patients’ diet and other influencing factors,it was found that 73% of the patients had1-3 food inducements and 5 median food inducements.2.The three most common food factors inducing or aggravating symptoms were spicy food(69.2%),cold drink(53.8%)and fried food(40.4%),which were related to seasonal alternation(5.8%),cold(26.9%),staying up late(23.1%)and eating out(34.6%).3.The abundance and diversity of flora in tongue coating group of IBS-D patients were higher than those in feces group(P < 0.05).4.The common dominant bacteria in stool and tongue coating samples of IBS-D patients were Bacteroides(64.66% vs 33.60%),Hardwall(24.20%vs 30.81%)and Proteus(7.81% vs 20.99%).The abundance of Bacteroides in both groups was the highest.5.The common dominant bacteria in stool and tongue fur samples of IBS-D patients were Puccinia,accounting for 18.47% and 18.86%respectively.6.At the generic level,correlation analysis showed that Clostridium F was strongly positively correlated with Veronococcus S and Alistipes,and negatively correlated with Parabacteroides.Veronococcus in group F was strongly negatively correlated with Johnsonella and actinomycetes in group S.Lactobacillus in group F was strongly positively correlated with Parasutterella,Sutterela and Parabacteroides in group S.Alistipes of group F Bifidobacterium belonged to group S was strongly negatively correlated.Conclusion1.The occurrence and aggravation of IBS-D are closely related to diet,and there are many food-induced or aggravated symptoms in IBS patients.The three most common food factorsthat induce or aggravate IBS-D patients’ symptoms include spicy stimulating foods,cold drinks and fried foods.They are also related to seasonal alternation,cold,staying up late and eating out.2.The abundance and diversity of flora in tongue coating group of IBS-D patients were higher than those in feces group.Moreover,microbes in different parts may interact with each other in the occurrence of diseases.