Correlation Study Of FOXA1 On Prostate Cancer Via The Immunohistochemstry And Bioinformatics Analysis

Objective: Based on the previous exon-sequencing of 26 sample Guangxi prostate cancer(PCa),to study the effect of FOXA1 on PCa.Methods: Eighteen clinical paired PCa and four cases prostatitis samples were utilized to examine the expression of FOXA1 by immunohistochemistry.The significance of FOXA1 was analyzed based on 493 PCa and 52 non-cancerous tissues from The Cancer Genome Atlas(TCGA)database.A total of 141 PCa and 73 non-cancerous tissues were available from the Oncomine database to explore the expressing profiles of FOXA1 in PCa.The cBioPortal and Gene Expression Profiling Interactive Analysis(GEPIA)were applied for obtaining FOXA1 co-expression genes,then using The Search Tool for The Retrieval of Interacting Genes/Proteins(STRING)to create protein-protein interaction networks.Moreover,the enrichment and potential pathway of FOXA1 were accomplished via Gene Oncology(GO)and KyotoEncyclopedia of Genes and Genomes(KEGG).GraphPad Prism 5.0 conducted correlation analysis.Results: FOXA1 was overexpressed in the nucleus of PCa(p<0.001),and it was significantly higher than non-cancerous tissues(P<0.001)by immunohistochemistry.TCGA and Oncomine data displayed that FOXA1 was remarkably over-expressed in PCa compared with that in non-PCa samples(p<0.001).The GO and KEGG revealed that FOXA1,AR and SIRT1 were significant in functional annotations(p<0.05).Moreover,the expression of FOXA1,AR and SIRT1 was positively correlated(p<0.0001)from TCGA.Conclusion: FOXA1 is overexpressed in PCa,which may affect PCa by indirectly regulating the expression of AR and SIRT1.FOXA1 might be a predictive marker for PCa onset and progress.