The Clinical Research Of Platelet MtDNA Levels In Patients With Inflammatory Bowel Disease

Introduction:Inflammatory bowel disease(IBD)is a chronic autoimmune disease characterized by non-specific inflammation of the intestinal mucosa.The etiology is still unclear.Intestinal immunity dysfunction is Related to IBD The immune system is consisted of innate immunity and acquired immunity.Currently,immune mechanisms are exerted through two damage-related molecular patterns,PAMPS and DAMPS.Studies have shown that mitochondrial DNA(mtDNA)can trigger Inflammation by recognizing TLRs in Immune cells.Platelets can maintain the hypercoagulable state of IBD and play an important role in the pathogenesis of IBD.What’s more Platelets activated are capable of releasing mtDNA.Whether intestinal mucosal damage is Related to mtDNA that origins of Platelets,and the correlation between mtDNA and cytokines is unclear.Objective:To investigate the clinical significance of platelet mtDNA in the changes of copies in patients of inflammatory bowel disease(IBD).Methods : This study enrolled67 patients with IBD from August 2017-March2018 including 33 UC patients and 34 CD patients.At the same time,16 cases of the healthy control group were selected with all normal results of medical biochemistry and imaging check in the near future,and nearly 1 month with no symptoms of gastrointestinal tract.Mitochondrial gene ND1 concentrations were measured by real-time quantitative PCR in the DNA extracted from plasma and platelet.Then the difference in copies of mtDNA(ND1)in three groups were analyzed.The levels ofplasma IL-1,IL-6 and TNF-αin IBD were measured by ELISA assays.Then the correlation between mtDNA and cytokines were analyzed.Results:MtDNA levels in platelet and plasma will be elevated in UC and CD patients compared to healthy controls.The levels of mtDNA in UC with different severity were significant differences(P<0.05),The levels of mtDNA in UC with different lesions areas were all no significant differences(P>0.05),in addition to the level of platelet mtDNA between E1(Rectum colon)and E2(left colon)group(P<0.05)..The levels of mtDNA in CD with different severity were significant differences(P<0.05),The levels of mtDNA in UC with different lesions and biological behavior areas were all no significant differences(P>0.05).The expression of mtDNA’s copies in platelets of IBD were significantly correlated with erythrocyte sedimentation rate(ESR),C-reactiveprotein(CRP)and DAI scores.MtDNA levels in plasma will be elevated in UC and CD patients compared to healthy controls.The levels of mtDNA in UC with different severity were significant differences(P<0.05),The levels of mtDNA in UC with different lesions areas were all no significant differences(P>0.05),in addition to the levels between E1(Rectum colon)and E3(extensive colon)group(P<0.05)..The levels of mtDNA in CD with different severity were significant differences(P<0.05),The levels of mtDNA in CD with different lesions and biological behavior areas were all no significant differences(P>0.05),in addition to the levels between L1(Rectum colon)and L3(extensive colon)group(P<0.05).The expression of mtDNA’s copies in plasma of IBD were significantly correlated with erythrocyte sedimentation rate(ESR),C-reactiveprotein(CRP)and DAI scores.The levels of plasma IL-1,IL-6 and TNF-αis elevated in UC and CD patients compared to healthy controls,which is positive correlation with plasma mtDNA.Conclusion: Platelet mtDNA as pro-inflammatory cytokine release induced in IBD patients,which provided new clues for futures diagnosis and treatment of IBD(P<0.05).