The Expression And Significance Of Serum TGF-beta 1 And Vascular Endothelial Growth Factor In Children With Congenital Heart Disease And Pulmonary Hypertension

Objective:Congenital heart disease(CHD)is the most common cause of birth defects in infants in China,which is caused by the incomplete development of cardiovascular system during fetal development.Pulmonary arterial hypertension(PAH)is one of the important causes of morbidity and mortality in children.Relevant studies have shown that about 10%of congenital children have pulmonary hypertension[1].Especially when Eisenmenger syndrome(ES)occurs,the morbidity and mortality of congenital heart disease with pulmonary hypertension will increase significantly[2].The etiology and pathogenesis of CHD complicated with PAH are still unknown.The purpose of this study is to discuss the value of serum transforming growth factor-beta(TGF-beta)and vascular endothelial growth factor(Vascular endothelial growth factor)to discuss its value in the pathogenesis of CHD with PAH,and to provide clues for early clinical intervention of CHD with PAHMethods:From September 2016 to June 2018,children with left-to-right shunt congenital heart disease were selected for outpatient treatment and hospitalization,and diseases of central nervous system,endocrine system and immune system were excluded.According to the mean pulmonary artery pressure estimated by color Doppler ultrasound,20 children with CHD and mild PAH,20 children with CHD and moderate PAH,and 20 children with CHD and severe PAH were selected as the experimental group.At the same time,20 healthy children in the physical examination center of our hospital were selected as healthy control group.No recent infection was found in healthy control group.Cardiac diseases were excluded by chest X-ray,electrocardiogram,cardiac ultrasound and laboratory examination,other serious organ diseases were excluded,primary pulmonary hypertension and pulmonary hypertension were excluded,and depression,schizophrenia and mania were exlcuded.1.The serum levels of TGF-beta 1 and VEGF were measured by ELISA.2.The serum levels of vascular endothelial growth factor were measured by ELISA.3.Statistical method:SPSS 19.0 software was used for data analysis.Results:1.Compared with the healthy control group,the serum levels of TGF-beta 1 and vascular endothelial growth factor in children with congenital heart disease and pulmonary hypertension increased significantly(P<0.05).2.Compared with the serum levels of TGF-beta 1 and VEGF,the serum levels of TGF-beta 1 and VEGF in healthy children were lower than those in mild congenital heart disease with pulmonary hypertension group,moderate congenital heart disease with pulmonary hypertension group and severe congenital heart disease with pulmonary hypertension group(P<0.05);the serum levels of TGF-beta in mild congenital heart disease with pulmonary hypertension group were lower than those in mild congenital heart disease with pulmonary hypertension group(P 1.The concentration of vascular endothelial growth factor in moderate congenital heart disease with pulmonary hypertension and severe congenital heart disease with pulmonary hypertension was higher than that in healthy control group and mild congenital heart disease with pulmonary hypertension group(P<0.05),and the difference was significant(P<0.05);the concentration of serum TGF-beta 1 and vascular endothelial growth factor in moderate congenital heart disease with pulmonary hypertension group was higher than that in healthy control group and mild congenital The serum levels of TGF-beta 1 and VEGF in severe congenital heart disease with pulmonary hypertension group were significantly higher than those in healthy control group,mild congenital heart disease with pulmonary hypertension group and moderate congenital heart disease with pulmonary hypertension group(P<0.05).Conclusion:1.TGF-beta 1 and VEGF may be involved in the occurrence of congenital heart disease with pulmonary hypertension2.The increase of pulmonary artery pressure in children with congenital heart disease and pulmonary hypertension may be related to the concentration of TGF-beta 1 and vascular endothelial growth factor.