Expression Pattern Of MicroRNA And Target Genes In Endometrium During Embryo Implantation

Objective: Infertility is a major problem faced by the current childbearing population under the influence of various factors.With the recent research on non-coding small RNAs,the role of mi RNAs in reproductive biology has gradually been discovered.Successful pregnancy requires high-quality embryos to work with the appropriate endometrium.Whether the embryo can be implanted into the endometrium determines the pregnancy outcome.Therefore,the crosstalk between the mother and the fetus has always been one of the important research directions in the field of reproductive medicine.During embryo implantation,mi RNAs have been found differentially expressed in Endometrium.This paper looks for target genes regulated by differentially expressed mi R-155 and mi R-146 a and looks for the expression of target genes,then explores the role of mi RNAs in endometrial receptivity.Methods: Based on mouse blastocyst implantation on the night of 4th day of gestation,this experiment established a pregnant mouse model,uterus was taken at the implantation window(days 4 and 5 of gestation)and pre-implantation(day 1 of gestation)..In order to exclude the influence of embryo,a pseudo-pregnant rat model was also taken,uterus was taken on tne days 4 and 5 of the pseudopregnancy and day 1 of the pseudopregnancy(pre-implantation period).The expression levels of mi RNA(mi R-155,mi R-146a)and m RNA(Sirt1,MMP16)in mouse endometrium were detected by Q-PCR.The expression of Sirt1 and MMP16 proteins in mouse endometrial tissues was detected by Western blotting and immunohistochemistry.To verify the regulation of mi RNA on its target gene,use mir-155/146 a agomir and has-mir-155 to overexpression mi RNA both in vivo and in vitro,respectively.Results: Compared with the pre-implantation period,the expressions of mir-155 and mir-146 a during the implantation window were low,accompanied by high expressions of Sirt1 and MMP16 proteins,showing a negative correlation.Mi R-155 and mi R-146 a were down-regulated in the endometrium of the embryo implantation site,and Sirt1 and MMP16 proteins were highly expressed,which was consistent with the above results.In vivo,overexpression of mi R-155 and mi R-146 a which inhibited the expression of Sirt1 and MMP16 proteins reflexively.In vitro,in the cell experiment,have the same results.Conclusion: mi R-155 and mi R-146 a,which are differentially expressed in the endometrial tissues of the implantation window,affect embryonic implantation by regulating the expression of Sirt1 and MMP16,regulating endometrial receptivity and cell invasion.The results of this study indicate that mi R-155/mi R-146 a regulates Sirt1 and MMP16 may be a molecular mechanism affecting endometrial receptivity.Targeting Sirt1 and MMP16 may be potential therapeutic strategies for recurrent spontaneous abortion and repeated implantation failure.