Effect Of Enteral Nutrition On Tight Junction Protein In Inflammatory Bowel Disease

Background: Inflammatory bowel disease(IBD)is a group of chronic recurrent diseases involving the intestinal tract.At present,there is no radical treatment method.The commonly used treatment methods in clinic either have large side effects or are expensive,which brings great psychological and economic burden to patients and their families.Glutamine is a necessary amino acid for intestinal mucosal cells.It can promote the proliferation of intestinal mucosal cells,repair intestinal barrier and maintain intestinal permeability.Studies have shown that supplementation of glutamine to IBD animal models and patients can promote the expression of intestinal tight junction protein and reduce intestinal permeability.Some scholars have found that when providing enteral nutrition in IBD patients,not only nutritional status has improvement,but alleviate the illness.In recent years,more and more studies have shown that enteral nutrition solution can induce and maintain disease remission to some extent,but the specific mechanism is unclear.The aim of this study was to investigate the effects of enteral nutrition on intestinal permeability,disease activity and intestinal tight junction protein in mice with inflammatory bowel disease,and to explore the possible therapeutic mechanism of enteral nutrition solution,so as to provide a new direction for the treatment of IBD.Objective:To explore the effects of enteral nutrition solution on intestinal permeability,disease activity and intestinal tight junction protein in IBD mice,and to provide a possible theoretical basis for the treatment of IBD with enteral nutrition solution.Methods: Trinitrobenzene sulfonic acid(TNBS)enema was used to prepare mice IBD model.After 7 days of enema,normal saline,normal saline,glutamine and enteral nutrition solution were given to normal and experimental mice respectively for 5 days.During the experiment,the mice's spirit,activity,diet and stool were recorded and observed.After 5 days,the serum of each group was obtained.The expression of Dlactic acid and Tumor necrosis factor-?(TNF-?)were detected by ELISA.After the mice were killed,the intestinal tissues of mice were obtained.The appearance of intestinal wall,the shape and length of intestinal lumen were observed.The structure of lower intestinal wall was further observed by HE staining under a microscope.Western blot and immunohistochemistry were used to detect the expression and distribution of tight junction protein Claudin-1 and Occludin in intestinal tissue of mice in normal control group,experimental control group,glutamine and enteral nutrition solution group.Result:(1)After 7 days of TNBS enema,the mice in the experimental group showed mental depression,weight loss,reduced food intake,decreased activity,decreased coat color and luster,incomplete stool,increased stool frequency,but no obvious mucopurulent stool.(2)After 5 days of continuous administration of glutamine and enteral nutrient solution to IBD mice,their mental state improved,their body weight and activity increased,their fur color brightened and their stool formed.(3)Intestinal wall congestion and edema were observed in the model group.HE staining showed that the intestinal wall of normal mice was intact,and the cells arranged neatly without inflammatory cell infiltration.Inflammatory infiltration and intestinal wall structural integrity in the intervention group of glutamine and enteral nutrition solution were better than those in the experimental group,but the intestinal tissue of the control group was still not as good as that of the control group.(4)The expression of intestinal tight junction protein Claudin-1 and Occludin in the model mice was significantly lower than that in the control group(P < 0.05).(5)The expression of intestinal tight junction protein Claudin-1 and Occludin in glutamine and enteral nutrition solution group was significantly higher than that in model group(P<0.05).Conclusion:(1)TNBS could induce inflammatory bowel disease model in mice,and the expression of intestinal tight junction protein Claudin-1 and Occludin in IBD mice decreased.(2)Glutamine and enteral nutrition solution can reduce the infiltration of inflammatory cells and improve the degree of inflammatory activity to a certain extent.(4)Glutamine and enteral nutrition solution can improve the expression of intestinal tight junction protein Claudin-1 and Occludin to some extent.