Experimental Study On Treatment Of TPH And Sert In Hippocampus、Cortex And Hypothalamus Of Rats With IBS Deficiency Of Spleen And Deficiency Syndrome By Tongxieyaofang

Objective : To observe changes of TPH and SERT in hippocampus,cortex and hypothalamus while IBS liver-depression and spleen-deficiency rats were treated by Tongxie Yaofang.And then to discuss its therapeutic mechanism.Methods:120 rats were divided randomly into normal group,model control group,low doses of Tongxie Yaofang group,middle doses of Tongxie Yaofang group,high doses of Tongxie Yaofang group and western medicine group.Each group was treated by restraint stress(for 14 days)and pouring belly with fanxieye(for 7 days)to make IBS liver-depression and spleen-deficiency models except the normal group.Then these models were treated by pouring belly with 5g Tongxie Yaofang(low doses of Tongxie Yaofang),10 g Tongxie Yaofang(middle doses of Tongxie Yaofang),15 g Tongxie Yaofang(high doses of Tongxie Yaofang)and Pinaverium Bromide for 7days.After the experiment was finished,rats’ prefrontal cortex,hypothalamus and hypothalamus were separated.And the actual concentration of TPH was calculated by the way of determining the OD value with ELIASA,while the SERT was analyzed by the way of ploymerase chain reaction(PCR).Results:The result of TPH: Rats’ hypothalamus TPH of pathological group is higher than that of normal group significantly(P<0.01),and the contents of TPH of different dose Tongxie Yaofang group are downturn than that of pathological group,the contents of hippocampus in low and high doses group decline markedly(P < 0.05),low group’s cortexdecline markedly(P<0.05),and middle group’s hypothalamus decline markedly(P<0.05).Taken together,the group of low doses Tongxie Yaofang is more effectively.The result of SERT: Rats’ hippocampus,cortex and hypothalamus 5-HT transporter of model group is lower than that of normal group significantly(P<0.01),and the contents of SERT in different doses of Tongxie Yaofang is higher than that of model group.Taken together,the groups of middle and high doses Tongxie Yaofang are more effectively(P<0.05 or P<0.01),and the change of western group rats’ contents of SERT makes no statistically significant to that of model group(P>0.05).Conclusion : 1)Spain by reducing the secretion of TPH,cortex,hippocampus,hypothalamus,increase the synthesis of SERT,make the corresponding part of the content of 5-HT into balance and thus reduce the liver to the stimulation of intestines and stomach to ease irritable bowel syndrome liver spleen deficiency rats gastrointestinal movement abnormalities.2)The effect of mental stimulation on the gastrointestinal tract is greater than that of gastrointestinal tract stimulation on mind.3)The brain gut peptide represented by 5-HT is probably one of the main material bases of Tongxie Yaofang in treating IBS of liver depression and spleen deficiency.