Therapeutic Effect Of Tongxie Yaofang On Patients With Diarrhea-predominant Irritable Bowel Syndrome And Its Effect On The Expressing Of IL-1β And IL-10

Purpose:The expression of IL-1β and IL-10 in sigmoid mucosa of patients with diarrheapredominant irritable bowel syndrome(IBS-D)was detected by immunohistochemistry.To observe the changes of Interleukin-1β(IL-1β)and Interleukin-10(IL-10)expression in t he sigmoid colon mucosa before and after taking the drug,and objectively evaluate the efficacy of Tongxie Yaofang in treating this disease;Immunohistochemical expression of IL-1β and IL-10 in sigmoid colonmucosa and the pathogenesis of IBS-D in liver stag nation and spleen deficiency syndr-ome.Methods:Seventy patients with IBS-D with liver stagnation and spleen deficiency included in the inclusion criteria.They were divided into two groups by random number table,w ith 35 cases in each group.One group in the western medicine group treated with pioa vi-roninm,and the other in the chinese medicine group treated with Tongxie Yaofang.35 healthy checkers were selected as the herlthy group according to the herlthy criteria(Finally each group fell off in 2 cases).And before and after 4 weeks treatment,accord ing to the symptom classification and quantification criteria;sigmoid mucosa of the hea lthy group and the sigmoid colon mucosa before and after 4 weeks treatment in the western medicine group and the Chinese medicine group were observed.Immunohistoc hemical expression of IL-1β and IL-10.Results:One:Compared with the symptom scores of the patients in the Chinese medicine group and the western medicine group,the symptom scores of the two groups were significantly improved after treatment,and the difference was statistically significant(p<0.05).The symptom score of the Chinese medicine group was significantly lower than that of the western medicine group.Statistically significant(p<0.05);the total effective rate of the two groups in the Chinese medicine group was higher than that in the western medicine group,thedifference was statistically significant(p<0.05).It is indicated that Tongxie Yaofang is effective in treating liver stagnation and spleen deficiency syndrome IBS-d.Two:The expression of IL-1β in the healthy group and the western medicine grou p an-d the Chinese medicine group were compared.The expression of IL-1β in the hea lthy group was less than that in the western medicine group and the traditional Chine se m-edicine group,and the difference was statistically significant(P<0.05),indicating th at IB-S-The occurrence of D may be related to the high expression of IL-1β in the sigmo-id colon mucosal immunohistochemistry.There was no significant difference in t he ex-pression of IL-1β immunohistochemistry between the western medicine group an d the traditional Chinese medicine group(P>0.05),which was comparable;The expres sion of IL-10 in the healthy group and the western medicine group and the traditional Chi-nese medicine group were compared.The expression of IL-10 in the healthy gro up wa-s higher than that in the western medicine group and the traditional Chinese m edicine group,and the difference was statistically significant(P<0.05),indicating that I BS-T-he occurrence of D may be related to the low expression of anti-inflammatory factor IL-10 in the sigmoid mucosal immunohistochemistry.There was no significant d ifferen-ce in the expression of IL-10 immunohistochemistry between the western medic ine gr-oup and the traditional Chinese medicine group(P>0.05).Comparability.Three:Compared with Western medicine group and Chinese medicine group,the expression of IL-1β in the healthy group was significantly different from that in the western medicine group and the traditional Chinese medicine group(P>0.05).There was no significant difference in the expression of IL-1β immunohistochemistry between the Chinese medicine group and the control group(P>0.05).The expression of IL-10 in the healthy group and the western medicine group and the Chinese medicine group were compared.There was no significant difference between the western medicine group and the traditional Chinese medicine group(P>0.05).There was no significant difference in the expression of IL-10 between the western medicine group and the traditional Chinese medicine group(P>0.05).It is suggested that the mechanism of action of the two treatment groups may be achieved by regulating the expression of IL-1β and IL-10 in the sigmoid colon.Conclusion:Tongxie Yaofang is effective in treating patients with IBS-D of liver stagnation and spleen deficiency syndrome.The mechanism may be associated with down–regulation of the the expression of IL-1β and up-regulation of the expression IL-10 in sigmoid colon mucosa.