Relationship Between JAM-1 And STAT3 And PSTAT3 In Patients With Ulcerative Colitis

Objective1.By detecting the expression of tight junction protein JAM-1 in patients with ulcerative colitis and normal intestinal mucosa,to analyze the changes in JAM-1protein expression in the intestinal mucosa of patients with ulcerative colitis.2.By detecting the expression of STAT3 and pSTAT3 in patients with ulcerative colitis and normal intestinal mucosa,to analyze the changes of STAT3 and pSTAT3 protein expression in the intestinal mucosa of patients with ulcerative colitis.3.To analyze the correlation between tight junction protein JAM-1 and STAT3 and pSTAT3.MethodsThe specimens were collected by electronic colonoscopy in the endoscopy room of our hospital from January 2017 to January 2018.The patients were diagnosed with ulcerative colitis by colonoscopy and pathological diagnosis.The diagnostic criteria refer to the Consensus Opinion on the Diagnosis and Treatment of Inflammatory Bowel Disease(2012).After signing the informed consent forms,40 specimens(The biopsy site of the specimen is the area with obvious inflammation of the colon under colonoscopy.)were collected for the experimental group,At the same time,20 cases of normal intestinal mucosa were collected in the operating room of our hospital as the control group(Gastrointestinal surgery Intestinal resection margin is >5cm away from the tumor,and the marginal is normal tissue with negative pathological,all the normal intestinal mucosa were the same source).Histopathological grades were used to assess the severity of ulcerative colitis.Immunohistochemistry(IHC)was used to detect the expression of JAM-1,STAT3 and pSTAT3 in colon tissue.Gastrointestinal surgery Intestinal resection margin is >5cm away from the tumor,and the marginal pathological section is negative normal tissueResults1.Immunohistochemistry results showed that the intensity of JAM-1 staining in the colonic mucosa of the experimental group was weaker than that of the control group,the number of stained cells was decreased,and the distribution was irregular.The intensity of STAT3 staining did not change significantly compared with the control group.the intensity of pSTAT3 staining was significantly enhanced,and the number of stained cells increased.It is suggested that the expression of tight junction protein JAM-1 protein in colonic mucosa of patients with ulcerative colitis is significantly lower than that of normal control group.the expression of pSTAT-3 is significantly higher than that of normal control group,and there is no significant difference in total STAT3 expression.2.Statistical results showed that compared with the control group,the expression of JAM-1 protein in the colonic mucosa of the experimental group decreased(P<0.05),and the expression of pSTAT3 increased(P<0.05).There was no statistically significant difference in the expression of total STAT3.3.The intestinal tight junction protein JAM-1 was negatively correlated with the expression of pSTAT3 in patients with ulcerative colitis as the severity of the disease changed.Conclusion1.The expression of JAM-1 protein in colonic mucosa of patients with ulcerative colitis is significantly lower than that of normal control group,which suggests that there may be destruction of intestinal mucosal barrier function in patients with ulcerative colitis.2.The expression of pSTAT3 in the colonic mucosa of patients with ulcerative colitis was significantly higher than that of the normal control group,which suggests that STAT3 protein phosphorylation is involved in the inflammatory process of UC.3.In patients with ulcerative colitis,JAM-1 is negatively correlated with the expression of pSTAT3 as the severity of the disease changes,which suggests that UC inflammation is accompanied by destruction of the intestinal mucosal barrier.