| Purpose:The dissertation is to observe the effect of Changningfang enema on serum inflammatory cytokines and colonic mucosal mechanical barrier of ulcerative colitis model rats,and to explore the possible mechanism of Changningfang in the treatment of ulcerative colitis.Material and method:Random to 60 weight(190 +10)g,6-8 weeks of SPF SD male rats were divided into building module(50)and blank group(10).Module is to be built and random model group,the mesalazine group,the Changningfang high group,the Changningfang medium group and the Changningfang low group,a total of 5 groups,each group of10.Building 3 days after the successful start medication,according to the surface area of conversion to determine the drug dose,the blank group and the model group was given saline enema 2 ml/time,the mesalazine group was given the mesalazine enema solution using 0.42g/kg of enema,the Changningfang high group,the Changningfang medium group,the Changningfang medium group water decoction enema,give the dose of 6.88 g/kg,3.44 g/kg,1.72 g/kg,the treatment for seven days in a row.After treatment,all rats fasted and could not restrain water for 24 h.After anesthesia,the rats were sacrificed by blood sampling from the abdominal aorta.The changes of colonic mucosal tissues were observed by naked eyes and light microscopy.ELISA method to detect the serum IL-6 and IL-10 levels,RT-q PCR method to detect colon tissue of Occludin m RNA,Claudin-2 m RNA and ZO-1 m RNA level.Results:1.After retention enema treatment with Changningfang,the amount of food and water,mental state,hair color glossiness,activity,body weight,feces and perianal mucus adhesion of rats in Changningfang groups were improved to varying degrees compared with model group.2.The gross observation of the colonic mucosal injury in rats: colonic mucosa injury of rats compared with the blank group,model group rats colon tissue injury score significantly higher(P < 0.01);Compared with model group,the treatment group rats colon tissue damage evaluation were lower(P < 0.05).3.Observation of the model group under light microscope showed that the lamina propria glands of the colonic mucosa were atrophy,arranged disorderly,and the morphology and distribution of goblet cells were changed.There were a large number of inflammatory cell infiltrations in the mucosal layer and submucosa,and necrotic edema and concealment were seen in the involved tissues and surroundings.The pit was destroyed;rats in each treatment group have different degrees of improvement.In the colon mucosa of the rats,inflammatory cell infiltration could be seen,the lamina propria gland structure was incomplete,the number of goblet cells was reduced,and the crypts were irregular.4.ELISA results: The results showed that:(1)Compared with the blank group,the content of IL-6 in the serum of the model group was significantly higher(P < 0.01);(2)Compared with the model group,serum IL-6 content in Changningfang groups and the mesalazine group was significantly decreased(P < 0.01);(3)Compared with the Changningfang high group,there was no statistical significance between the Changningfang high group and the mesalazine group(P > 0.05),and there was no statistical significance between the different doses of Changningfang groups(P > 0.05).The results showed that:(1)Compared with the blank group,model group rats serum IL-10 content decreased significantly(P < 0.01);(2)Compared with model group,Changningfang each dose group and western medicine group content of IL-10 in serum of rats were increased(P < 0.05);(3)The Changningfang high group compared with the mesalazine group,there was no statistically significant difference(P > 0.05),compared with a group from the Changningfang,there was no statistically significant difference(P > 0.05),compared with the Changningfanglow group,the difference was statistically significant(P < 0.05).5.RT-PCR results: T-PCR detection of Occludin gene expression in colon of rats showed that:(1)Compared with the blank group,the expression of Occludin gene in colon of rats in the model group was significantly decreased(P < 0.01);(2)Compared with the model group,the expression of Occludin gene in colon of rats in Changningfang groups and the mesalazine group increased(P < 0.05);(3)Compared with the Changningfang high group,there was no statistical significance between the Changningfang high group and the mesalazine group(P >0.05),and there was no statistical significance between the different doses of Changningfang groups(P > 0.05).RT-PCR detection of ZO-1 gene expression in the colon of rats showed that:(1)Compared with the blank group,the expression of ZO-1 gene in the colon of rats in the model group was significantly decreased(P < 0.01);(2)Compared with model group,the expression of ZO-1 gene in colon of rats in Changningfang groups and the mesalazine group was increased(P < 0.05);(3)The difference between the Changningfang high group and the Changningfang medium group and the Changningfang low group was statistically significant(P < 0.05).There was no statistical difference between the Changningfang high group and the mesalazine group(P > 0.05).There was no statistical difference between the Changningfang middle group and the Changningfang low group(P > 0.05).RT-PCR was used to detect the expression of Claudin-2 gene in the colon of rats.(1)Compared with the blank group,the expression of Claudin-2 gene in the colon of rats in the model group was significantly increased(P < 0.01).(2)Compared with model group,the expression of Claudin-2 gene in colon of rats in Changningfang groups and the mesalazine group was decreased(P < 0.05);(3)Compared with the Changningfang high group,there was no statistical significance between the Changningfang high group and the mesalazine group(P > 0.05),and there was no statistical significance between the different doses of Changningfang groups(P > 0.05).Conclusion:Changningfang for ulcerative colitis model rats has a certain therapeutic effect,its mechanism may be related to reduce inflammation,repair intestinal epithelial tissue is closely connected,prompting intestinal mucosal permeability is reduced,thus protect the intestinal mucosal barrier function. |
PDF Full Text Request