| Millettia speciosa Champ.is a plant of the Millettia genus belonging to the Leguminosae family.It has the effect of mending the lungs and activating the strong gluten.Mainly used for lumbar muscle strain,rheumatoid arthritis,and cure lung fever,lung deficiency cough,tuberculosis,chronic bronchitis,chronic hepatitis,spermatozoa,Leucorrhea and so on.Objective:The purpose of this project is to systematically study the quality standards of the southern drug Millettia speciosa,and to formulate a strong quality evaluation method for cattle.Methods:1?The base source,characters,microscopic characteristics and TLC identification of Millettia speciosa were carried out in-depth and meticulous identification of raw medicines.2?According to a series of requirements in the Chinese pharmacopoeia,the water content,total ash content,acid insoluble ash and four kinds of leaching objects of different batches of Millettia speciosa were measured.The content of five heavy metals was measured in the above different batches of Millettia speciosa samples,and five heavy metal pollution indices were calculated.In addition,more than five years of Millettia speciosa detection items,as well as the comparison of Millettia speciosa five different parts of the above detection items.3?The optimum extraction method of total flavonoids from Millettia speciosa was investigated by using the RSM,and the content of total flavonoids in different batches of Millettia speciosa samples was determined by the chromogenic method,and the content of total flavonoids in five different years was determined.and compare the total flavonoids content in five different parts of Millettia speciosa.The content of total polysaccharide in different batches of Millettia speciosa samples was determined by phenol-sulfuric acid method,and the content of total polysaccharide in five different years of Millettia speciosa.and compare the total polysaccharide content in five different parts of Millettia speciosa.4?The content of Paulownia,Quercetin,Fermononetin and Maackiain in different batches of Millettia speciosa samples was determined by HPLC,and the content of four kinds of ingredients in five different years of Millettia speciosa was determined.And the content of more than four components in five different parts of Millettia speciosa were determined by comparison.5?The fingerprint of 27 batches of Millettia speciosa was studied,and the fingerprint of Millettia speciosa was established under specific extraction conditions.Through fingerprint,22 groups of common peaks were obtained,and the similarity of 27 groups of cattle in large samples was calculated.6?The content of 16 amino acids and 10 kinds of trace elements in different batches of Millettia speciosa samples were determined,and the corresponding results were analyzed and studied.At the same time,the content of 16 amino acids and trace elements in five different years were determined,the content of amino acids and the content of trace elements in five different parts of Millettia speciosa were determined,and the content of amino acids and the content of trace elements in Millettia speciosa were evaluated by SPSS analysis of the above experimental data.Results:1?The basis for the identification of Millettia speciosa pharmacognosy was determined.2?To determine the water content of Millettia speciosa medicinal materials,it is recommended that the water content does not exceed 11%.To determine the total ash content of Millettia speciosa medicinal herbs,it is suggested that the total ash content should not exceed 6.5%.To determine the acid insoluble ash content of Millettia speciosa medicinal materials,it is suggested that the content of acid insoluble ash should not exceed 0.55%.To determine the content range of four kinds of leaching objects of Millettia speciosa medicinal materials,it is suggested that the content of hot water leaching is not less than 11%,the content of hot alcohol leaching is not less than 4.5%,the content of cold water leaching is not less than 7.5%,and the content of cold alcohol leaching is not less than 2%.To determine the range of comprehensive pollution index caused by five heavy metals of Millettia speciosa medicinal materials.3?The optimum extraction condition of total flavonoids was 40?,the ethanol concentration was 60%,the material ratio was 1:25,and the extraction time was 30min.The total flavonoids content is between 0.042%?0.551%.The total flavonoids content of 3 years of raw cattle was the highest.The total flavonoids content of the old leaves was the highest,at 2.508%.The optimum extraction condition of total polysaccharide content in Millettia speciosa is that the temperature is 5?,the extraction time is 30min,and the material liquid ratio is 1:20.The total polysaccharide content of Millettia speciosa medicinal herbs is between 3.13%?8.64%.The total polysaccharide content of 3 years of raw cattle was the highest.The total polysaccharide content of flowers was the highest,at 6.88%.4?The content of paulownia in Millettia speciosa medicinal is between 0.081g/kg and 0.358g/kg.The content of quercetin in Millettia speciosa medicinal is between 0.232mg/kg and 31.037mg/kg.The content of Fermononet in Millettia speciosa medicinal is between 1.612mg/kg and 263.785mg/kg.The content of Maackiain in Millettia speciosa medicinal is between 0.379mg/kg and 246.97mg/kg.The content of 2-year-old Millettia speciosa paulownia was the highest,0.358g/kg.The contents of quercetin,fermononetin and maackiain were the highest in 3 years,respectively,17.830mg/kg,37.247mg/kg and 87.715mg/kg.The content of quercetin in the branches was the highest,17.384mg/kg,and the flowers had the highest content of the fermononetin,which was 5.475mg/kg.and maackiain only in the rhizome and new leaves,the content of the new leaves is higher than the rhizome.The paulownia does not exist in the old leaves,new leaves,branches and flowers,but only in the rhizome.5?27 batches of Millettia speciosa sample similarity in more than 9.6?The results of amino acid clustering analysis were as:The ? group was S4,S17,S10,S27,S30,S13,S19,S15,S20,S29.The ? group is S6,S28,S9,S14.The ? group is S5,S7,S22,S1,S11,S24,S23,S18,S21,S16,S8,S12,S2,S3,S25,S26.The results of trace element clustering analysis are as:? group is S3,S7,S23,S2,S5,S1.The ? group is S27.? group is S17,S19,S16,S18,S20,S29,S6.The ? group is S4,S26,S10,S11,S9,S25,S8,S12,S15,S24,S14,S28,S13,S21,S22,S30.Conclusion:1?The transverse and microscopic tectonic features of Millettia speciosa roots can be used as the main identification basis for Millettia speciosa medicinal materials.2?The moisture,total ash,acid insoluble ash,leaching and heavy metals of Millettia speciosa medicinal materials can be used as the main reference basis for their quality evaluation..3?Taking rutin as the control product,it is feasible to use aluminum nitrate coloring method to determine the total flavonoids of cattle under specific extraction conditions,and the total flavonoids of cattle can be used as the evaluation index of Millettia speciosa quality,and glucose is the control product,It is feasible to use phenol-sulfuric acid method to determine the total polysaccharide of Millettia speciosa obtained by specific treatment method under specific extraction conditions,and the total polysaccharide of Millettia speciosa can be used as an evaluation index of the quality of it.4?The content range of paulownia,quercetin,fermononetin and maackiain can be used as evaluation indexes for the quality of Millettia speciosa medicinal materials.5?Under this extraction condition,the similarity degree of Millettia speciosa HPLC fingerprint is good.6?The clustering analysis of Millettia speciosa amino acids and trace elements shows that the total amount of amino acids in Millettia speciosa increases with the increase of growth years,and the better the appearance traits,the more amino acids. |
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