| Objective To study the protective effect of panax notoginseng polysaccharide on hepatic ischemia reperfusion injury in rats and to explore the mechanism of reducing hepatic ischemia reperfusion injury.Method33 male SD rats were randomly selected from 3 to make ischemia reperfusion models of left and middle liver.After successful modeling,the remaining 30 SD rats were randomly divided into 3 groups: control group,model group and panax notoginseng polysaccharide group,with 10 rats in each group.In the control group,only the abdominal cavity was opened and the hepatoduodenal ligaments were exposed.Before the operation,the model group was given 10 ml normal saline once a day for 7 consecutive days.The first hepatic hilum was exposed during the operation,and the blood flow into the left and middle lobe of the liver was blocked by vascular clamp for 45 min,followed by the release of vascular clamp and reperfusion for 4h.The panax notoginseng polysaccharide group was given 10 ml of panax notoginseng polysaccharide once a day for 7 consecutive days before the operation.The operation method was the same as the model group.Samples were collected after perfusion for the following tests: changes in serum aminotransferase(AST and ALT)in each group of rats were determined.Detect each group rats serum inflammatory indexes(TNF-α and IL-1β);The activities of malonone(MDA)and superoxide dismutase(SOD)in liver were detected.Liver histopathological changes were observed under microscope.Expression levels of ho-1 and TLR4 in liver tissues of rats in each group were determined by IHC and WB.Results1.the model group rats serum ALT(426.86± 35.89),AST(457.34± 42.78),TNF-α(137.14± 15.48)and IL-1 β(185.09± 17.34)and control group ALT(28.75± 4.53),AST(32.49± 3.25),TNF-α(28.16± 3.57)and IL-1β(25.47± 4.13)significantly increased(P < 0.01),and notoginseng polysaccharides group ALT(291.16± 24.73),AST(237.49± 18.36),TNF-α(81.73±7.34),IL-1β(105.68 ±10.84)significantly decreased,compared with the model group had significant difference(P < 0.01).2.the model group rats liver tissue SOD activity(197.24 ± 10.48)than control group in SOD activity(257.85 ± 17.56)significantly decreased(P < 0.01),MDA(4.49 ± 0.28)activity was MDA(2.47 ± 0.24)activity increased significantly(P < 0.01);and notoginseng polysaccharides group SOD(234.40± 15.37)increased significantly,compared with model group(P < 0.01),MDA(3.59 ± 0.17)significantly decreased,compared with the model group had significant difference(P < 0.01).3.Liver histopathological changes: the liver tissue structure of the control group was clear,and there was no degeneration and necrosis of liver cells;In the model group,hepatic tissue structure was disordered,hepatocyte swelling and degeneration were obvious,and hepatocyte necrosis was observed in different degrees.The degree of hepatocyte swelling,degeneration and necrosis in panax notoginseng polysaccharide group was significantly reduced compared with the model group.4.The expression of ho-1 and TLR4 in rat liver tissues detected by IHC and WB showed that the level of ho-1 in the model group was significantly higher than that in the control group(P<0.01),and that in the panax notoginseng polysaccharide group was significantly higher than that in the model group(P<0.01).The TLR4 level in the model group was significantly higher than that in the control group(P<0.01),while the TLR4 level in the panax notoginseng polysaccharide group was significantly lower than that in the model group(P<0.01).Conclusion1.Panax notoginseng polysaccharide can reduce the levels of ALT and AST in the serum of rat liver ischemia reperfusion injury,reduce the pathological damage degree of rat liver tissue,and improve the liver ischemia reperfusion injury of rat.2.The role of panax notoginseng polysaccharide in alleviating hepatic ischemia reperfusion injury in rats may be related to up-regulation of HO-1 and down-regulation of TRL4 expression in liver tissues,as well as the reduce of infammatory responses and oxidative stress. |
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