Experimental Study On Magnetic Resonance Imaging Of Glioma Using Porous Iron Oxide Nanoparticles

Part I Prearation of porous iron oxide nanoparticles and its in vivo and vitro MR imagingObjective:To synthesize and characterize Porous iron oxide and to verify the enhancement effect of iron oxide nanoparticles in magnetic resonance imaging T2WI sequence of in-vitro.Establishing orthotopic glioblastoma xenografts model of mice,detecting the T2WI enhancement effect of in-vivo glioma mediated by nano-iron oxide.Methods:Porous iron oxide was prepared by chemical synthesis method,and its porous and hollow structure was proved by transmission electron microscopy and scanning electron microscopey.T2WI imaging of iron oxide with different concentration gradients was performed by high field intensity magnetic resonance to prove its T2WI enhancement effect.Stereotactic locator combined with microinjector intracranial injection of U87 glioma cell suspension was used to establish orthotopic glioblastoma xenografts model.After successful modeling,iron oxide was injected through the tail vein for T2WI scanning,and the signal changes of glioma precontrast and postcontrast were observed.Conclusion:Porous nano-iron oxide prepared by chemical synthesis method has uniform particle size,porous and hollow structure.In vitro magnetic resonance imaging of Porous iron oxide has good T2WI enhancement effect,through the tail vein inject it into mice with orthotopic glioblastoma xenografts model can significantly reduce tumor tissue signal,which is a prospective magnetic resonance contrast agent with drug loading function.Part II monocytes labeling of Porous iron oxide nanoparticles in vitroObjective:Measuring the effect of Porous iron oxide on the survival rate and migration ability of monocytes and labeling monocytes with Porous iron oxide in vitro.Magnetic resonance T2WI imaging was performed on the labeled cells.T2 time of monocytes labeled with nano-iron oxide at different concentrations were measured to determine the optimal labeling concentration.Methods:MTT method were used to measure the cytotoxicity of Porous iron oxide,cell migration experiment test were used to test the effects of nano-iron oxide on monocytes migration.Using different concentrations of Porous iron oxide incubate with monocytes,Prussian blue staining test whether iron oxide nanoparticles were take up by monocytes.collecting The co-cultured cells for T2WI and T2mapping imaging to measure T2 time.Conclusion:Porous iron oxide has little toxicity to monocytes within a certain concentration range and does not affect their migration ability.In vitro labeled monocyte magnetic resonance imaging has a good T2WI enhancement effect and can be used as a carrier for glioma targeted imaging and tumor treatment.