Analysis Of Gut Microbiose In Patients With Colorectal Cancer And Adenoma

ObjectiveColorectal cancer is a common malignant tumors of digestive system,and it ranks third for cancer incidence and mortality worldwide.Early detection and treatment are important methods to protect against colorectal cancer.Intestinal microbiota dysbiosis could lead to various diseases.Recent studies have reported that altered intestinal microbiota may play an important role in the progression of colorectal cancer.But the pathogenesis of colorectal cancer is still not entirely clear.In this study,the relative abundance of fecal microbiota was validated by real-time quantitative PCR to investigate the changes gut microbiota in patients with colorectal cancer and adenoma,and to explore the value of microbiota dysbiosis in development of colorectal cancer.Methods:We measured the relative abundance of Lactobacillus,Bifidobacterium,Bacaeroides,Bacteroides fragilis,Enterotoxigenic Bacteroides fragilis,Fusobacterium nucleatum,and Peptostreptococcus anaerobius by quantitative PCR in fecal samples from patients with colorectal cancer,colorectal adenoma and healthy individuals.We evaluated the relationship between gut microbiota and colorectal cancer and adenoma.Results:1.The relative quantification of Lactobacillus,Bifidobacterium,Bacaeroides in patient of colorectal adenoma and colorectal cancer have no significant difference compared with the healthy control group.The relative quantification of Fusobacterium nucleatum,Peptostreptococcus anaerobius,were increased in colorectal cancer patients than healthy group and colorectal adenoma patients,and the relative quantification of Enterotoxigenic Bacteroides fragilis were increased in colorectal adenoma patients compared with healthy group.2.The relative quantification of Peptostreptococcus anaerobius is associated with tumor stage.Detection of Fusobacterium nucleatum and Peptostreptococcus anaerobius is expected to be a microbial marker for non-invasive screening of colorectal cancer and adenoma.Conclusions:Intestinal microbiota dysbios may correlated with colorectal cancer and colorectal adenoma,and it’s expected to serve as a microbial marker for non-invasive screening of colorectal adenoma and colorectal cancer.