Studies On The Expression Of Long Non-coding RNA-H19 In Colonic Tissue Of The Diarrhea-predominant Irritable Bowel Syndrome Patients With Liver Depression And Spleen Deficiency Syndrome

Objective The changes of intestinal mechanical barrier function were investigated by observing the ultrastructural changes of microvilli,and tight connections in colon tissues in IBS-D patients with liver depression and spleen deficiency syndrome;The expression levels of lnc RNA-H19 in colon tissue of IBS-D patients with liver depression and spleen deficiency syndrome and healthy volunteers were measured to explore the differences in the expression and distribution levels.Methods 1.Colonic tissue samples of the patients who met the diagnostic criteria of IBS-D with liver depression and spleen deficiency syndrome and the healthy subjects were included in the outpatient department of gastroenterology and the inpatient department of the first affiliated hospital of Zhejiang university of traditional Chinese medicine,the microvilli and tight connections of Colonic tissues were observed by transmission electron microscope.2.Lnc RNA-H19 expression levels in colon tissues of patients and healthy control group were detected by q RT-PCR,and the differences and distribution levels between them were compared.Results 1.Under electron microscope,the colonic tissues of IBS-D patients with liver depression and spleen deficiency syndrome showed irregular microvilli with different lengths,widened tight connections,decreased desmosomes,and no obvious changes in mitochondria and other structures.2.Lnc RNA-H19 expression levels in colon tissue samples of patients in the IBS-D group with liver depression and spleen deficiency syndrome was lower than that in the control group(P<0.05).Conclusion In IBS-D patients with liver depression and spleen deficiency syndrome,the microvilli in the colon were irregular,with different lengths,wider tight connections and fewer desmosomes,indicating that the intestinal mechanical barrier function was impaired.; Lnc RNA-H19 expression was decreased in colon tissues of IBS-D patients with liver depression and spleen deficiency syndrome,which may be involved in the occurrence of diarrhea-predominant irritable bowel syndrome with liver depression and spleen deficiency type.